FIGURE SUMMARY
Title

Expression of Insl3 Protein in Adult Danio rerio

Authors
Donizetti, A., Calicchio, M., Romano, M.Z., Rosati, L., Turco, M., Carrese, A.M., Del Gaudio, R., Ferrandino, I., Aniello, F.
Source
Full text @ Int. J. Mol. Sci.
Figure 1

Gene expression pattern for insl3 gene by RT-PCR analysis. (A) RT-PCR gel image. (B) Relative statistical analysis of insl3 expression in zebrafish tissues. Data were normalized with rplp0 cDNA and reported as OD ratio. All values are expressed as means ± SEM. *: p ≤ 0.05; ns: not significant.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Adult
Figure 2

Protein expression pattern of Insl3 by Western blotting analysis. (A) Western blot and (B) relative statistical analysis of Insl3 protein levels in Danio rerio tissues. Data were normalized with α-Tubulin and reported as OD ratio. All values are expressed as means ± SEM. **: p ≤ 0.01; ****: p ≤ 0.0001.

EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage: Adult
Figure 3

Immunohistochemistry for Insl3 in Danio rerio testis. The immunolocalization signal appears as brown areas. (A–E): Antibody positivity is evident in Spermatogonia A (SPG_A), Spermatogonia B (SPG_B), Spermatocytes I (SPC I), Spermatocytes II (SPC II), and Spermatids (SPT) and Spermatozoa (SPZ). The low signal is evident in the LC (asterisks) and no positivity is found in the Sertoli cells (arrow). The negative control sections ((D) inset) show no signal. The scale bars correspond to 50 µm in the figure (D) insert, 20 µm in figure (A), and 10 µm in figures (B–E).

EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage: Adult
Figure 4

Analysis of Insl3 and Rxfp2 sequences in vertebrate species. (A) Sequence alignment of the Insl3 protein Homo sapiens Insl3 (NP_005534.2); Rattus norvegicus Insl3 (NP_446132.1); Gavialis gangeticus Insl3 (XP_019379679.1); Anser cygnoides Insl3 (XP_047908400.1); Rana temporaria Insl3 (XP_040177352.1); Danio rerio Insl3 (NP_001108525.2); Polypterus senegalus Insl3 (XP_039623679.1); and Callorhinchus milii Insl3 (AFP04465.1). (B) Sequence alignment of the Rxfp2 extracellular domain. Homo sapiens (NP_570718.1); Rattus norvegicus (NP_001012493.1); Gavialis gangeticus (XP_019381047.1); Anser cygnoides (XP_013044814.2); Rana temporaria (XP_040196386.1); Danio rerio Rxfp2b (NP_001315011.1); Danio rerio Rxfp2a (NP_001315313.1); Polypterus senegalus (XP_039622298.1); and Callorhinchus milii (XM_00789132.1). Identical amino acids are indicated by asterisks; conservative substitutions are shown by colons and semiconservative substitutions by dots. Different colors represent the physiochemical properties of amino acids.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.
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