FIGURE SUMMARY
Title

Role of the Melanocortin System in Gonadal Steroidogenesis of Zebrafish

Authors
Navarro, S., Crespo, D., Schulz, R.W., Ge, W., Rotllant, J., Cerdá-Reverter, J.M., Rocha, A.
Source
Full text @ Animals (Basel)
Figure 1

Relative expression levels of melanocortin-related genes in zebrafish ovaries and testes. Expression of the 18s rRNA gene was used as the reference gene for normalization of cDNA levels. Asterisk indicates significant variation after a two-way ANOVA analysis followed by Sidak’s multiple comparison test (*** p < 0.001, **** p < 0.0001). Data are expressed as mean ± SEM.

EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Adult
Figure 2

Localization of mcr1 and mcr4 transcripts expression in the zebrafish ovary by in situ hybridization. Panels (A,B) show mc1r and mc4r expression in the follicular cells, respectively. Panels (C,D) display mc1r and mc4r expression in the previtellogenic and vitellogenic follicles of the zebrafish ovary. (E,F) are representative tissue sections of (C,D) stained with haematoxylin-eosin, respectively. Signal was absent in the interstitial region.

EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Adult
Figure 3

Localization of mcr1 and mcr4 transcripts expression in zebrafish testes by in situ hybridization. Panels (A,B) show mc1r and mc4r expression in germ cells, respectively. (C,D) are magnified views of (A,B) for detail. (E,F) are representative tissue sections of (A,B) stained with toluidine blue, respectively.
Figure 4

Effects of melanocortin peptides on basal and human chorionic gonadotropin (hCG)-induced estradiol (E2) secretion in previtellogenic and vitellogenic ovarian follicles of zebrafish. (A) Effects of ACTH, (B) MSH chemical analogues (MTII), (C) human ASIP, and (D) chemical antagonist (SHU9119). Experiments were performed in triplicate and repeated at least three times. Data is expressed as a percentage of the control (mean ± SEM). Different letters indicate significant variations after a one-way ANOVA analysis, followed by Tukey’s post-hoc test, p < 0.05.
Figure 5

Effect of melanocortin peptides on basal and human chorionic gonadotropin (hCG)-induced testosterone (T) in primary cell cultures of dispersed testicular cells of zebrafish. (A) Effects of ACTH, (B) MSH chemical analogues (MTII), (C) human ASIP and (D) chemical antagonist (SHU9119). Experiments were performed in triplicate and repeated at least three times. Data is expressed as a percentage of the control (mean ± SEM). Different letters indicate significant variations after a one-way ANOVA analysis, followed by Tukey’s post-hoc test, p < 0.05.
Acknowledgments
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