ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Positive correlation between NIPBL and RUNX1 expression in megakaryocytes derived from healthy donors and in bone marrow cells derived from 34 adult AML patients. A and B, Spearman's correlation between RUNX1 and RAD21 (A) or NIPBL (B) in cord blood megakaryocytes (MK) derived from healthy donors. C and D, Spearman's correlation between RUNX1 and RAD21 (C) or NIPBL (D) in bone marrow cells (BM) derived from 34 adult AML patients without aberrant RUNX1 alterations (mutations or translocations). E and F, Spearman's correlation between RUNX1 and NIPBL in 34 adult AML patients without (NPM1wt) (E) or with NPM1 mutation (NPMc+) (F). Spearman's correlation analysis showed a significant positive correlation of the ratio of RUNX1 expression only versus NIPBL, not versus RAD21,. r = Spearman's correlation coefficient

runx1 expression is specifically reduced following nipblb down‐regulation. A‐C WISH analyses of runx1 expression at the stage of 30 hpf in embryos injected with control morpholino (ctrl‐MO) (A), nipblb‐MO (B) and nipblb‐MO with runx1‐mRNA (C). The runx1 expression in the caudal region (higher magnification in the box) is reduced following nipblb down‐regulation and rescued in embryos co‐injected with nipblb‐MO and runx1 mRNA. E‐F, RT‐qPCR analyses of the P1runx1 (D) and P2runx1 (E) isoforms in ctrl‐MO‐, nipblb‐MO‐ and nipblb‐MO/runx1mRNA‐injected embryos at 48 hpf. Scale bars indicate 100 μm. One‐way ANOVA with Bonferroni correction, ^**P < .01, ^*P < .05, n.s: non‐significant

NIPBL‐mediated RUNX1 down‐regulation leads to impaired expression of RUNX1 target genes in both human and zebrafish. A, Spearman's correlation between RUNX1 and MPL in cord blood megakaryocytes (MK) derived from healthy donors. B, Spearman's correlation between RUNX1 and SPI1 in bone marrow cells (BM) derived from 34 adult AML patients without aberrant RUNX1 alterations (mutations or translocations). r = Spearman's correlation coefficient. C and D, RT‐qPCR analyses of 48 hpf ctrl‐, nipblb‐ and nipblb‐MO/runx1mRNA‐injected embryos. C, The expression of the erythroid marker gata1a was decreased following nipblb‐MO injection in comparison with controls and rescued in nipblb‐MO/runx1mRNA‐injected embryos. D, The expression of the myeloid marker spi1b was increased in both nipblb‐MO‐ and nipblb‐MO/runx1mRNA‐injected embryos in comparison with controls. E‐G, O‐dianisidine staining showed a reduction of mature circulating erythrocytes in nipblb‐MO‐injected embryos at 48 hpf in comparison with ctrl‐MO. Co‐injection with the full‐length runx1 mRNA rescues the o‐dianisidine reduction. Lateral views anterior to the left (upper panels) and ventral views of the anterior region (lower panels). H‐J, WISH analyses showed an increased expression of spi1b in nipblb‐MO‐ and nipblb‐MO/runx1mRNA‐injected embryos in comparison with ctrl‐MO. Scale bars indicate 100 μm in (E‐G) and 200 in μm in (H‐J). One‐way ANOVA with Bonferroni correction, ^***P < .001 ^**P < .01, ^*P < .05, n.s: non‐significant

The down‐regulation of rad21 in zebrafish enhances the expression of runx1 and its downstream targets gata1a and spi1b. A‐D, RT‐qPCR analyses of 48 hpf ctrl‐ and rad21‐MO‐injected embryos. The expression of both P1 (A) and P2runx1 (B) isoforms and of runx1 targets gata1a (C) and spi1b (D) was increased following rad21‐MO injection in comparison to  controls at 48 hpf. One‐way ANOVA with Bonferroni correction, ^***P < .001, ^**P < .01, ^*P < .05, n.s: non‐significant