Green tea extract (GTE) suppressed lipid accumulation in zebrafish larvae. Live zebrafish at 30 dpf (day post fertilization) with a standard length of approximately 10 mm were used. (a) Experimental design of the zebrafish obesogenic (ZO) test. (b) Lateral view under a fluorescence microscope after Nile Red staining. vat, visceral adipose tissue (VAT); sb, swim bladder. (c) Representative VATs from live larva were selected after Nile Red staining, and images were recorded before and after 24 h of exposure or not to GTE (100 μg/mL). Nile Red-positive areas indicate VAT (red). (d) Quantification of the intensity of Nile Red positive staining. GTE reduced the amount of VAT compared to that in animals on a high fat diet (control). The Y axis indicates the ratio of Nile Red staining before and after GTE treatment for 24 h. * p < 0.05 vs. control, n = 5, error bars indicate SD.

GTE reduced VAT and decreased plasma triglycerides (TG) and total cholesterol (TCHO) levels in adult obese zebrafish. (a) Diagram of the overfeeding experiment. GTE administration and overfeeding started on week 0 and week 2, respectively. (b) Typical images of normal-feeding (NF) and overfeeding (OF) zebrafish with or without GTE. (c) Change in body weight during the feeding experiment. (d) GTE reduced VAT in OF zebrafish. GTE suppressed the increase of plasma TG (e) and TCHO (f) in OF zebrafish. * p < 0.05, ** p < 0.01, n = 5, error bars indicate SD.

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.