ZFIN Image: Zang et al., 2019, Figure 1

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Figure 1

Green tea extract (GTE) suppressed lipid accumulation in zebrafish larvae. Live zebrafish at 30 dpf (day post fertilization) with a standard length of approximately 10 mm were used. (a) Experimental design of the zebrafish obesogenic (ZO) test. (b) Lateral view under a fluorescence microscope after Nile Red staining. vat, visceral adipose tissue (VAT); sb, swim bladder. (c) Representative VATs from live larva were selected after Nile Red staining, and images were recorded before and after 24 h of exposure or not to GTE (100 μg/mL). Nile Red-positive areas indicate VAT (red). (d) Quantification of the intensity of Nile Red positive staining. GTE reduced the amount of VAT compared to that in animals on a high fat diet (control). The Y axis indicates the ratio of Nile Red staining before and after GTE treatment for 24 h. * p < 0.05 vs. control, n = 5, error bars indicate SD.

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