FIGURE SUMMARY
Title

Functional characterization of CNOT3 variants identified in familial adenomatous polyposis adenomas

Authors
Delacruz, R.G.C., Sandoval, I.T., Chang, K., Miller, B.N., Reyes-Uribe, L., Borras, E., Lynch, P.M., Taggart, M.W., Hawk, E.T., Vilar, E., Jones, D.A.
Source
Full text @ Oncotarget
Fig. 2

CNOT3 is required for intestinal differentiation. WISH staining for (A) the gut precursor marker gata6 (pg) at 48 hpf and (B) the differentiated gut-specific marker fabp2 (g) in 72 and 96 hpf control and cnot3a morphants.

 

EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth
Fig. 3

Functional characterization of CNOT3 variants. (A) The lordosis phenotype (red arrow) caused by cnot3a knockdown was rescued by co-injection of 250 pg wildtype human CNOT3 (hCNOT3) with 4 ng cnot3a morpholino (mo). (B) Percentage of rescue after co-injection of hCNOT3, E20K, or E70K variants with cnot3a mo (n > 75 per group). Significance of co-injection rescue was determined using Fisher’s exact test compared to cnot3a mo injection alone. ns = not significant. ****= p-value < 0.0001.

 
Fig. 4

Human CNOT3 rescues intestinal defects in zebrafish apcmcr embryos. (A) Graph shows cnot3a mRNA expression relative to 18s in 72 hpf embryos. (B) apcmcr and wildtype embryos, WISH staining for gut marker fabp2 for vehicle and hCNOT3 injected apcmcr embryos. (C) WISH staining for hCNOT3 E20K and E70K-injected apcmcr embryos and vehicle-injected apcWT. Note that the red arrowhead = fabp2 staining. (D) Percentage of apcmcr fish with gut marker staining from (A and B) at 72 hpf (n > 10 per group). Statistical significance of CNOT3 mRNA injection rescue was determined using Fisher’s exact test compared to uninjected apcmcr embryos alone. ns = not significant; *** = p-value = 0.0003; **= p-value = 0.0043.

 

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage: Protruding-mouth
Fig. 5

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth
Fig. S1

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Adult
Fig. S2

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Protruding-mouth
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Oncotarget
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