FIGURE SUMMARY
Title

Ligustilide, a major bioactive component of Angelica sinensis, promotes bone formation via the GPR30/EGFR pathway

Authors
Yang, F., Lin, Z.W., Huang, T.Y., Chen, T.T., Cui, J., Li, M.Y., Hua, Y.Q.
Source
Full text @ Sci. Rep.
Fig. S1

Effects of ligustilide on bone formation in zebrafish. (a) Bright field image of zebrafish at 9 dpf. (b) The mRNA expression of spp1, alp, runx2a, sp7, and bmp2b measured by quantitative RT-PCR in zebrafish at 9 dpf. Error bars indicate S.E.M. (n = 3).

 
Figure 1

LIG increases bone mass in vivo. (a) Calcein staining of zebrafish larvae at 9 dpf. Scale bars, 500 μm. Arrowheads, bone calcein staining. (b) Number of calcein-stained vertebrae in zebrafish at 9 dpf. Error bars indicate s.e.m., n = 13 for each group. ###P ≤ 0.001 vs. control group. **P ≤ 0.01, ***P ≤ 0.001 vs. model group. (c) The expression mRNA for Alp, Runx2a, sp7, and BMP2b were measured by quantitative RT-PCR in zebrafish at 9 dpf. Error bars indicate s.e.m. (n = 4). #P ≤ 0.05, ###P ≤ 0.001 vs. control group. *P ≤ 0.05, **P ≤ 0.01 vs. model group.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.
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