FIGURE SUMMARY
Title

Neuronal expression patterns of the PlexinA family during zebrafish development

Authors
Emerson, S.E., Light, S.E., Ebert, A.M.
Source
Full text @ Gene Expr. Patterns
Fig. 2

plxnA1a expression in the developing zebrafish.

Brightfield images of zebrafish embryos processed for in situ hybridization. (A-F) Whole-mount lateral, (A’-F’) whole-mount dorsal. Brightfield sections (A’’-F’’) forebrain, (A’’’-F’’’) midbrain and (C’’’’-F’’’’) hindbrain. Embryos were imaged at different developmental time points. (A-A’’’) 18 hpf, (B-B’’’) 24 hpf, (C-C’’’’) 36 hpf, (D-D’’’’) 48 hpf, (E-E’’’’) 60 hpf, and (F-F’’’’) 72 hpf. Lines in (A-F) indicate locations of the sections shown at that time-point. Inset shows sense probe control. Hpf-hours post fertilization, OpV- optic vesicles, FBr-forebrain, V- ventricle, NR-neural retina, L-lens, TeO- optic tectum, OV- otic vesicle, CMZ-cilliary marginal zone, lTeO- lateral optic tectum, MO- medulla oblongata, IPL-inner plexiform layer, RGC- retinal ganglion cell layer, FB- fin bud.
Fig. 3

plxnA1b expression in the developing zebrafish.

Brightfield images of zebrafish embryos processed for in situ hybridization. (A-F) Whole-mount lateral, (A’-F’) whole-mount dorsal. Brightfield sections (A’’-F’’) forebrain, (A’’’-F’’’) midbrain and (C’’’’-F’’’’) hindbrain. Embryos were imaged at different developmental time points. (A-A’’’) 18 hpf, (B-B’’’) 24 hpf, (C-C’’’’) 36 hpf, (D-D’’’’) 48 hpf, (E-E’’’’) 60 hpf, and (F-F’’’’) 72 hpf. Lines in (A-F) indicate locations of the sections shown at that time-point. Inset shows sense probe control. Hpf-hours post fertilization, OpV- optic vesicle, Fbr, forebrain, SC- spinal cord, NR-neural retina, V- ventricle, HB- hindbrain, OV- otic vesicle, L-lens, Hi-intermediate hypothalamus, lfb-lateral forebrain bundle, Po-pre-optic region, T-thalamus, lTeO- lateral optic tectum, MO- medulla oblongata, RGC- retinal ganglion cell layer, IPL-inner plexiform layer, CG-cranial ganglia.
Fig. 4

plxnA2 expression in the developing zebrafish.

Brightfield images of zebrafish embryos processed for in situ hybridization. (A-F) Whole-mount lateral, (A’-F’) whole-mount dorsal. Brightfield sections (A’’-F’’) forebrain, (A’’’-F’’’) midbrain and (C’’’’-F’’’’) hindbrain. Embryos were imaged at different developmental time points. (A-A’’’) 18 hpf, (B-B’’’) 24 hpf, (C-C’’’’) 36 hpf, (D-D’’’’) 48 hpf, (E-E’’’’) 60 hpf, and (F-F’’’’) 72 hpf. Lines in (A-F) indicate locations of the sections shown at that time-point. Inset shows sense probe control. Hpf-hours post fertilization, OpV- optic vesicle, HG-hatching gland, CMZ-cilliary marginal zone, DT-dorsal thalamus, V3- 3rd ventricle, Hi-intermediate hypothalamus, OV- otic vesicle, oc-optic chiasm, RGC- retinal ganglion cell layer, INL-inner nuclear layer, T-thalamus, PTv-ventral part of posterior tuberculum, lTeO- lateral optic tectum, MO- medulla oblongata.
Fig. 5

plxnA3 expression in the developing zebrafish.

Brightfield images of zebrafish embryos processed for in situ hybridization. (A-F) Whole-mount lateral, (A’-F’) whole-mount dorsal. Brightfield sections (A’’-F’’) forebrain, (A’’’-F’’’) midbrain and (C’’’’-F’’’’) hindbrain. Embryos were imaged at different developmental time points. (A-A’’’) 18 hpf, (B-B’’’) 24 hpf, (C-C’’’’) 36 hpf, (D-D’’’’) 48 hpf, (E-E’’’’) 60 hpf, and (F-F’’’’) 72 hpf. Lines in (A-F) indicate locations of the sections shown at that time-point. Inset shows sense probe control. Hpf-hours post fertilization, LF- lateral forebrain, NR-neural retina, MO- medulla oblongata, DT-dorsal thalamus, PTv-ventral part of posterior tuberculum, Po-pre-optic region, TeO- optic tectum, T-thalamus, OV- otic vesicle, R-retina.
Fig. 6

plxnA4 expression in the developing zebrafish.

Brightfield images of zebrafish embryos processed for in situ hybridization. (A-F) Whole-mount lateral, (A’-F’) whole-mount dorsal. Brightfield sections (A’’-F’’) forebrain, (A’’’-F’’’) midbrain and (C’’’’-F’’’’) hindbrain. Embryos were imaged at different developmental time points. (A-A’’’) 18 hpf, (B-B’’’) 24 hpf, (C-C’’’’) 36 hpf, (D-D’’’’) 48 hpf, (E-E’’’’) 60 hpf, and (F-F’’’’) 72 hpf. Lines in (A-F) indicate locations of the sections shown at that time-point. Inset shows sense probe control. Hpf-hours post fertilization, OV- otic vesicle, NR-neural retina, L-lens, lfb-lateral forebrain bundle, TeO- optic tectum, PTd-dorsal part of posterior tuberculum, IPL-inner plexiform layer, T-thalamus, DT-dorsal thalamus, PTv-ventral part of posterior tuberculum, Po-pre-optic region, and MO- medulla oblongata, oc-optic chiasm, OG-otic ganglion, FB- fin bud, HG-hatching gland.
Fig. S2

plxna3 trunk expression.

Whole-mount in situ hybridization showing plxnA3 expression. A. Lateral, A’’. Dorsal and A’’’. Dorsal-zoom views at 24 hpf.
Acknowledgments
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Reprinted from Gene expression patterns : GEP, 27, Emerson, S.E., Light, S.E., Ebert, A.M., Neuronal expression patterns of the PlexinA family during zebrafish development, 56-66, Copyright (2017) with permission from Elsevier. Full text @ Gene Expr. Patterns
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