Fig. 1

Protein sequence alignments, phylogenetic tree, and expression patterns of zebrafish diras1a and diras1b. a Multiple alignments of diras1a and diras1b amino acid sequences were compared with those of human (H-DI-Ras1), mouse (M-DI-Ras1), rat (R-DI-Ras1), and Xenopus (X-DI-Ras1). The DIRAS1 sequences from the five species were aligned using ClustalW. Identical sequences are shaded in yellow, whereas residue similarities of the four DIRAS1 proteins are presented in cyan. b A phylogenetic tree was constructed based on the multiple alignments of the DIRAS1 proteins using MegAlign in DNASTAR through the neighbor-joining method. Scale bars indicate nucleotide substitutions (100×). c A graphical representation of the conserved synteny of the DIRAS1 gene clusters in human chromosome 19, rat chromosome 7, mouse chromosome 10, and zebrafish linking group (LG) 2. The diras1a gene is localized in LG 11. Ch denotes the chromosome. Whole mount in situ hybridization was performed using antisense riboprobes against diras1a (d) and diras1b (e) at the indicated developmental stages. The stages of embryonic development are indicated in the right (d, e). di diencephalon, GCL ganglion cell layer, H.B. hindbrain, and tel telencephalon. Stages are indicated in the upper right
Fig. 6

Effects of diras1 gene depletion on neurogenesis using the Tg(huC:gfp) model. The embryos that received the indicated MOs were collected at 48 hpf. The expression of huC was abrogated in diras1 morphants. Reduced trigeminal sensory ganglion territories were also observed in diras1 morphants. The deficiency in huC expression was restored via co-injection with the wild-type but not with the C terminus truncated form of mouse Diras1 mRNA. All images are shown in lateral view. Scale bars 100 μm. Tc telencephalic cluster, Tg trigeminal ganglion, Tec tectum, Hb hindbrain
Fig. 7

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Fig. 8

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Fig. 9

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Fig. S3

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Mol. Neurobiol.
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