- Title
-
Unexpected Neuronal Protection of SU5416 against 1-Methyl-4-Phenylpyridinium Ion-Induced Toxicity via Inhibiting Neuronal Nitric Oxide Synthase
- Authors
- Cui, W., Zhang, Z., Li, W., Mak, S., Hu, S., Zhang, H., Yuan, S., Rong, J., Choi, T.C., Lee, S.M., and Han, Y.
- Source
- Full text @ PLoS One
SU5416 protects against MPTP-induced TH+ region area decrease in zebrafish. One dpf zebrafish embryos were co-incubated with 200 μM MPTP and SU5416, VRI or 0.3% DMSO (vehicle control) at the indicated concentrations for 2 days. After treatment, zebrafish were collected to perform whole-mount immunohistochemistry. (A) Representative pictures of whole-mount immunostaining of zebrafish brain from different treatment groups. (B) Magnification of diencephalic area of zebrafish larval (indicated by red bracket in Fig. 2A). (C) Statistical analysis of TH+ region area in each treatment group (20 fish embryos per group). Data, expressed as percentage of control, were the mean ± SEM of three separate experiments; ##p<0.01 versus control; *p<0.05 and **p<0.01 versus MPTP group (Turkey’s test). |
SU5416 increases the number of dopaminergic neurons in MPTP-treated zebrafish larval. One dpf zebrafish embryos were co-incubated with 200 μM MPTP and 1 μM SU5416 or 0.3% DMSO (vehicle control) for 2 days. After treatment, zebrafish were collected to perform paraffin-embedding, sectioning and immunostaining. (A) Representative picture of immunostaining of zebrafish section. (B) Statistical analysis of the number of TH-positive neurons in each treatment group (n = 12 fish/group). *p<0.05 versus MPTP group (Turkey’s test). |
Anti-angiogenic effects of SU5416 and VRI in zebrafish. One dpf Tg(fli-1:EGFP) transgenic zebrafish embryos were treated with SU5416, VRI or DMSO (vehicle control) at the indicated concentrations for 2 days. After treatment, intersegmental-vessel formations were observed under fluorescence microscopy. Deficit of blood vessels was indicated by yellow asterisks. |