Temporal expression pattern analysis of slc2a10 during development and in adult zebrafish tissues. RNA samples were purified from different embryonic and larval stages and from adult fishes and amplified with specific primer pairs of slc2a10. RT-PCR analysis (A) showed that two splice variants were expressed both maternally and at different developmental stages, similar to adult fish. Q-PCR (B) was used to quantify the expression levels of total slc2a10 mRNA during embryogenesis and in adult fishes. Expression of ef-1α housekeeping gene was used to normalize the relative expression levels of slc2a10 mRNA, which were compared to the corresponding values of the 1-cell stage (set to 1). Two specific PCR products of 727 and 550 bp were amplified from total RNA purified from adult zebrafish liver, heart, brain, eyes, gills, spleen, ovary, swim bladder, caudal fins, intestine, testes and kidney (C). To assess the RNA quality, a β-actin mRNA-specific product was obtained from all tissues analyzed. Abbreviations: A, adult; M, Size Marker.

Whole-mount in situ hybridization analysis of slc2a10 expression during zebrafish development. slc2a10 mRNA was ubiquitously distributed from the 1-cell stage to the 1k-cell stage (A,F) and during the gastrula period (G,H). At the 15-somite stage, a hybridization signal was localized in the otic placode and notochord (I); at the 20-25 somites stage, slc2a10 transcript was also expressed in the YSL region and in the notochord (J,K,L). During the pharyngula period (24-hpf), an intense signal was largely diffused in the cephalic region (M). In 48-hpf embryos (N,O), the slc2a10 mRNA was detected in the lens, posterior tectum, otic vesicles, hatching gland cells and pectoral fin buds. During the larval stage in 72-hpf embryos (P), slc2a10 expression was restricted to the otic vesicles and pectoral fins; in 4-dpf larvae (Q), the staining was observed in the swim bladder structure. Abbreviations: hgc, hatching gland cells; nt, notochord; pfb, pectoral fin buds; op, otic placode; ov, otic vesicles; sb, swim bladder; YSL, yolk syncytial layer.