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Fig. 4
Whole-mount in situ hybridization analysis of slc2a10 expression during zebrafish development. slc2a10 mRNA was ubiquitously distributed from the 1-cell stage to the 1k-cell stage (A,F) and during the gastrula period (G,H). At the 15-somite stage, a hybridization signal was localized in the otic placode and notochord (I); at the 20-25 somites stage, slc2a10 transcript was also expressed in the YSL region and in the notochord (J,K,L). During the pharyngula period (24-hpf), an intense signal was largely diffused in the cephalic region (M). In 48-hpf embryos (N,O), the slc2a10 mRNA was detected in the lens, posterior tectum, otic vesicles, hatching gland cells and pectoral fin buds. During the larval stage in 72-hpf embryos (P), slc2a10 expression was restricted to the otic vesicles and pectoral fins; in 4-dpf larvae (Q), the staining was observed in the swim bladder structure. Abbreviations: hgc, hatching gland cells; nt, notochord; pfb, pectoral fin buds; op, otic placode; ov, otic vesicles; sb, swim bladder; YSL, yolk syncytial layer.