Expression of zebrafish barhl in the developing central nervous system. (A–E) Dorsal views of 90%-epiboly embryos showing barhl2 expression in the anterior neural plate (black arrowheads), in relation to the expression domains of pax2 (blue arrowheads), emx1 (green arrowheads), rxb (yellow arrowheads) and flh (red arrowheads). Expression of these genes is summarised in E. Anterior to the top. Frontal (F,K,P,U; dorsal to the top), lateral (G,J,L,O,Q,T,W,X; anterior to the left) and dorsal (H,I,M,N,R,S,V,Y; anterior to the top) views of embryos showing expression of barhl2 (F–J), barhl1.1 (K–O), barhl1.2 (P–T), ngn1 (W), and a combination of immunohistochemistry against GFP in the tg(ngn-1-GFP) line together with whole mount in situ hybridisation for barhl1.1 (U,V) and barhl2 (X,Y). Stages are 10-somite (F,K,P), 18-somite (U,V), 24 hpf (G,L,Q,W,X,Y), 36 hpf (H,I,M,N,R,S), and 48 hpf (J,O,T). Abbreviations: cb (cerebellum), dt (dorsal thalamus), ep (epithalamus), hind (hindbrain), ph (posterior hypothalamus), pret (pretectum), pt (posterior tuberculum), ret (retina), tec (tectum), tel (telencephalon).

Overlapping domains of zebrafish barhl expression during late embryogenesis. Expression domains of barhl2 (A,B,C,C′), barhl1.1 (D,E,F,F′), and barhl1.2 (G,H,I,I′) at 60 h post-fertilisation, shown in dorsal (A,D,G; anterior to the left), frontal (B,C′,E,F′,H,I′ dorsal to the top) and lateral (C,F,I; anterior to the left) views. The border of the eye is depicted with a dashed line in C, F and I. Expression in the retina is shown in arrowheads. Abbreviations: e (eye), hind (hindbrain), pret (pretectum), ret (retina), tec (tectum).