- Title
-
Characterization of the retinoic acid receptor genes raraa, rarab and rarg during zebrafish development
- Authors
- Hale, L.A., Tallafuss, A., Yan, Y.L., Dudley, L., Eisen, J.S., and Postlethwait, J.H.
- Source
- Full text @ Gene Expr. Patterns
raraa (A, D–F), rarab, (C, G–I), and rarg (B, J–L) expression visualized at 1.5–2 hpf (A, B), 4.7 hpf (C), 9 hpf (D,G,J) and 10.5–11 hpf (E,F,H,I,K,L). Dorsal view (A,B,F,I,L). Lateral view; anterior up and dorsal to the left (D,E,G,H,J,K). (E) Grey arrowheads mark narrow anterior bands. Open black arrowhead marks posterior broad band. (H) Grey arrowhead marks single anterior band. Black arrowhead marks faint broad band. Open black arrowhead marks prominent broad band. Abbreviations: h, presumptive head; lm, lateral mesoderm; nt, notochord; tb, tailbud; v, vegetal pole; yp, yolk plug. Scale bar represents 118 μm in 3A, 3B, 135 μm in 3C, and 121 μm in 3D-L. EXPRESSION / LABELING:
|
raraa (A–E), rarab (F–J) and rarg (K–O) transcript expression visualized at 12 hpf (A,F,K), 24 hpf (B,C,G,H,L,M) and 48 hpf (D,E,I,J,N,O). Refer to text for detailed descriptions. Flat-mount embryos seen from a dorsal view (A,C,E,F,H,J,K,M,O) or a lateral view (B,D,G,I,L,N). Anterior is to the left. (A) Asterisk marks expression in two stripes lateral to the neural tube. (A–C) Open black arrowhead marks broad stripe of raraa hindbrain expression. (F,G) Open black arrowhead marks broad stripe of rarab hindbrain expression. (I) Inset shows a magnified view of rarab expression in the pharyngeal region. (K) Asterisk marks anterior lateral mesenchyme. (N) Grey arrowhead marks staining around eye. Abbreviations: c, cerebellum; e, eye; fb, forebrain; hb, hindbrain; nc, neural crest; ov, otic vesicle; pec, pectoral fin bud; pa, pharyngeal arches; phe, pharyngeal endoderm; sc, spinal cord; t, tectum; tb, tailbud. Scale bar represents 115 μm in 4A,F,K; 100 μm in 4B,G; 90 μm in 4L; 42 μm in 4C,H; 35 μm in 4D,E,I,J,N,O; 33 μm in 4M. EXPRESSION / LABELING:
|
Expression of raraa (A–D), rarab (E–H) and rarg (I–M). Refer to text for detailed descriptions. Anterior is to the left. Whole-mount embryos viewed dorsally (A,B,E,F). Flat-mount embryos seen from a dorsal view (C,D,G,H,J,K) or a lateral view (I,L,M). raraa expression and egr2b expression in the hindbrain at 11 hpf (A,B) and 24 hpf (C,D). Open black arrowhead marks anterior boundary of raraa expression in the posterior hindbrain (A,C,D). rarab and egr2b expression in the hindbrain at 11 hpf (E,F) and 24 hpf (G,H). Open black arrowhead marks anterior boundary rarab expression in the posterior hindbrain (E,F). Arrowhead marks the anterior boundary of rarab expression in medial hindbrain (G,H). Magnified view of rarg expression in the neurocranium and arches (I) and hindbrain striping (J) at 48 hpf. rarg tail expression at 10 hpf (K), 24 hpf (L) and 48 hpf (M). Abbreviations: ancr, anterior neurocranium; ba, branchial arches; c, cerebellum; nt, notochord; ov, otic vesicle; pa1, pharyngeal arch 1; pa2, pharyngeal arch 2; r3, rhombomere 3; r5, rhombomere 5; tb, tailbud. Scale bar represents 115 μm in 5A,B,E,F; 104 μm in 5C,D,G,H; 51 μm in 5I; 73 μm in 5J; 16 μm in 5K; 88 μm in 5L,M. EXPRESSION / LABELING:
|
Reprinted from Gene expression patterns : GEP, 6(5), Hale, L.A., Tallafuss, A., Yan, Y.L., Dudley, L., Eisen, J.S., and Postlethwait, J.H., Characterization of the retinoic acid receptor genes raraa, rarab and rarg during zebrafish development, 546-555, Copyright (2006) with permission from Elsevier. Full text @ Gene Expr. Patterns