TALEN

TALEN1-gjd2a

ID

ZDB-TALEN-170822-4

Name

TALEN1-gjd2a

Previous Names

None

Target

gjd2a (1)

Target Sequence 1

5' - GAGCAGCGATGGGAGAATG - 3'

Target Sequence 2

5' - GGACAGCAGCCTCCAAGAG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
fh437	gjd2a

Expression

Gene expression in Wild Types + TALEN1-gjd2a

No data available

Phenotype

Phenotype resulting from TALEN1-gjd2a

No data available

Phenotype of all Fish created by or utilizing TALEN1-gjd2a

Phenotype	Fish	Conditions	Figures
retinal outer plexiform layer Ab1-gjd2a labeling absent, abnormal	gjd2a^fh437/fh437	standard conditions	Fig. 6 from Quint et al., 2021
retinal pigmented epithelium increased thickness, abnormal	gjd2a^fh437/fh437	standard conditions	Fig. 1 from Quint et al., 2021
Mauthner neuron chemical synaptic transmission increased frequency, abnormal	gjd2a^fh437/fh437	standard conditions	Figure 4 from Lasseigne et al., 2021
Mauthner neuron cell communication by electrical coupling decreased magnitude, abnormal	gjd2a^fh437/fh437	mechanical stress	Figure 3 from Lasseigne et al., 2021
Mauthner neuron cell communication by electrical coupling decreased frequency, abnormal	gjd2a^fh437/fh437	standard conditions	Figure 4 from Lasseigne et al., 2021
retinal ganglion cell Ab1-gjd2a labeling absent, abnormal	gjd2a^fh437/fh437	standard conditions	Fig. 6 from Quint et al., 2021
retinal outer nuclear layer Ab1-gjd2a labeling absent, abnormal	gjd2a^fh437/fh437	standard conditions	Fig. 6 from Quint et al., 2021
Mauthner neuron chemical synaptic transmission normal occurrence, ameliorated	gjd2a^fh437/fh437	chemical treatment by environment: 6-Cyano-7-nitroquinoxaline-2,3-dione, chemical treatment by environment: 2-amino-5-phosphonopentanoic acid	Figure 4 from Lasseigne et al., 2021
Mauthner neuron synapse absent, abnormal	gjd2a^fh437/fh437	standard conditions	Fig. 2 from Shah et al., 2015
Mauthner neuron cell communication by electrical coupling decreased magnitude, abnormal	gjd2a^fh437/fh437	mechanical stress, chemical treatment by environment: 6-Cyano-7-nitroquinoxaline-2,3-dione, chemical treatment by environment: 2-amino-5-phosphonopentanoic acid	Figure 3 from Lasseigne et al., 2021
photoreceptor outer segment layer Ab1-gjd2a labeling absent, abnormal	gjd2a^fh437/fh437	standard conditions	Fig. 6 from Quint et al., 2021
eye refractivity, abnormal	gjd2a^fh437/fh437	standard conditions	Fig. 2 from Quint et al., 2021
lens decreased diameter, abnormal	gjd2a^fh437/fh437	standard conditions	Fig. 1 from Quint et al., 2021
retinal inner plexiform layer Ab1-gjd2a labeling absent, abnormal	gjd2a^fh437/fh437	standard conditions	Fig. 6 from Quint et al., 2021
Mauthner neuron chemical synaptic transmission absent process, abnormal	gjd2a^fh437/fh437	mechanical stress, chemical treatment by environment: 6-Cyano-7-nitroquinoxaline-2,3-dione, chemical treatment by environment: 2-amino-5-phosphonopentanoic acid	Figure 3 from Lasseigne et al., 2021
Mauthner neuron dendrite gjd1a expression decreased amount, abnormal	gjd2a^fh437/fh437; zf206Et	standard conditions	Figure 2 from Lasseigne et al., 2021
Mauthner neuron synapse ab1-tjp1 labeling decreased amount, abnormal	gjd2a^fh437/fh437; zf206Et	standard conditions	Figure 2 from Lasseigne et al., 2021
Mauthner neuron protein localization to synapse disrupted, abnormal	gjd2a^fh437/fh437; zf206Et	standard conditions	Figure 2 from Lasseigne et al., 2021
Mauthner neuron dendrite gjd2a expression decreased amount, abnormal	gjd2a^fh437/fh437; zf206Et	standard conditions	Figure 2 from Lasseigne et al., 2021
Mauthner neuron synapse gjd2a expression decreased amount, abnormal	gjd2a^fh437/fh437; zf206Et	standard conditions	Figure 2 from Lasseigne et al., 2021
Mauthner neuron synapse gjd1a expression decreased amount, abnormal	gjd2a^fh437/fh437; zf206Et	standard conditions	Figure 2 from Lasseigne et al., 2021
Mauthner neuron dendrite ab1-tjp1 labeling decreased amount, abnormal	gjd2a^fh437/fh437; zf206Et	standard conditions	Figure 2 from Lasseigne et al., 2021
afferent neuron synapse Ab1-gjd1a labeling absent, abnormal	gjd2a^fh437/fh437; zf206Et (AB/TU)	standard conditions	Fig. 5 from Miller et al., 2017
afferent neuron synapse Ab1-gjd2a labeling absent, abnormal	gjd2a^fh437/fh437; zf206Et (AB/TU)	standard conditions	Fig. 5 from Miller et al., 2017
startle response process quality, abnormal	gjd2a^fh437/fh437; zf206Et (AB/TU)	standard conditions	Fig. 8 from Miller et al., 2017
cranial nerve VIII synapse Ab1-gjd2a labeling absent, abnormal	gjd2a^fh437/fh437; zf206Et (AB/TU)	standard conditions	Fig. 5 from Miller et al., 2017
cranial nerve VIII synapse ab3-gjd2 labeling absent, abnormal	gjd2a^fh437/fh437; zf206Et (AB/TU)	standard conditions	Fig. 3 from Miller et al., 2017
afferent neuron synapse absent, abnormal	gjd2a^fh437/fh437; zf206Et (AB/TU)	standard conditions	Fig. 3 from Miller et al., 2017
spinal cord interneuron synapse absent, abnormal	gjd2a^fh437/fh437; zf206Et (AB/TU)	standard conditions	Fig. 3 from Miller et al., 2017
Mauthner neuron synapse non-functional, abnormal	gjd2a^fh437/fh437; zf206Et (AB/TU)	standard conditions	Fig. 4 from Miller et al., 2017
Mauthner neuron synapse assembly arrested, abnormal	gjd2a^fh437/fh437; zf206Et (AB/TU)	standard conditions	Fig. 3 from Miller et al., 2017
afferent neuron synapse assembly arrested, abnormal	gjd2a^fh437/fh437; zf206Et (AB/TU)	standard conditions	Fig. 3 from Miller et al., 2017
cranial nerve VIII synapse Ab1-gjd1a labeling absent, abnormal	gjd2a^fh437/fh437; zf206Et (AB/TU)	standard conditions	Fig. 5 from Miller et al., 2017
Mauthner neuron synapse ab3-gjd2 labeling absent, abnormal	gjd2a^fh437/fh437; zf206Et (AB/TU)	standard conditions	Fig. 3 from Miller et al., 2017
Mauthner neuron synapse Ab1-gjd1a labeling absent, abnormal	gjd2a^fh437/fh437; zf206Et (AB/TU)	standard conditions	Fig. 5 from Miller et al., 2017
Mauthner neuron synapse absent, abnormal	gjd2a^fh437/fh437; zf206Et (AB/TU)	standard conditions	Fig. 3 from Miller et al., 2017
spinal cord interneuron synapse Ab1-gjd2a labeling absent, abnormal	gjd2a^fh437/fh437; zf206Et (AB/TU)	standard conditions	Fig. 5 from Miller et al., 2017
afferent neuron synapse ab3-gjd2 labeling absent, abnormal	gjd2a^fh437/fh437; zf206Et (AB/TU)	standard conditions	Fig. 3 from Miller et al., 2017
spinal cord interneuron synapse assembly arrested, abnormal	gjd2a^fh437/fh437; zf206Et (AB/TU)	standard conditions	Fig. 3 from Miller et al., 2017
spinal cord interneuron synapse Ab1-gjd1a labeling absent, abnormal	gjd2a^fh437/fh437; zf206Et (AB/TU)	standard conditions	Fig. 5 from Miller et al., 2017
startle response latency, abnormal	gjd2a^fh437/fh437; zf206Et (AB/TU)	standard conditions	Fig. 8 from Miller et al., 2017
spinal cord interneuron synapse ab3-gjd2 labeling absent, abnormal	gjd2a^fh437/fh437; zf206Et (AB/TU)	standard conditions	Fig. 3 from Miller et al., 2017
Mauthner neuron synapse Ab1-gjd2a labeling absent, abnormal	gjd2a^fh437/fh437; zf206Et (AB/TU)	standard conditions	Fig. 5 from Miller et al., 2017
Mauthner neuron dendrite gjd1a expression decreased amount, abnormal	gjd1a^fh436/fh436; gjd2a^fh437/fh437; zf206Et	standard conditions	Figure 2 from Lasseigne et al., 2021
Mauthner neuron protein localization to synapse disrupted, abnormal	gjd1a^fh436/fh436; gjd2a^fh437/fh437; zf206Et	standard conditions	Figure 2 from Lasseigne et al., 2021
Mauthner neuron dendrite gjd2a expression decreased amount, abnormal	gjd1a^fh436/fh436; gjd2a^fh437/fh437; zf206Et	standard conditions	Figure 2 from Lasseigne et al., 2021
Mauthner neuron synapse gjd2a expression decreased amount, abnormal	gjd1a^fh436/fh436; gjd2a^fh437/fh437; zf206Et	standard conditions	Figure 2 from Lasseigne et al., 2021
Mauthner neuron synapse gjd1a expression decreased amount, abnormal	gjd1a^fh436/fh436; gjd2a^fh437/fh437; zf206Et	standard conditions	Figure 2 from Lasseigne et al., 2021
retina ab1-cx35 labeling absent, abnormal	gjd2a^fh437/fh437; gjd2b^fh454/fh454	standard conditions	Fig. S5 from Quint et al., 2021
retina Ab1-gjd2a labeling absent, abnormal	gjd2a^fh437/fh437; gjd2b^fh454/fh454	standard conditions	Fig. S5 from Quint et al., 2021
area postrema neuronal action potential increased rate of occurrence, abnormal	mitfa^w2/w2; gjd2a^fh437/fh437	chemical treatment by environment: Mefloquine hydrochloride	Figure 6 from Brunal et al., 2021
Mauthner neuron neuronal action potential increased rate of occurrence, ameliorated	mitfa^w2/w2; gjd2a^fh437/fh437	chemical treatment by environment: pentetrazol	Figure 1 from Brunal et al., 2021
rhombomere 7 neuronal action potential increased rate of occurrence, abnormal	mitfa^w2/w2; gjd2a^fh437/fh437	chemical treatment by environment: Mefloquine hydrochloride	Figure 6 from Brunal et al., 2021
area postrema neuronal action potential increased rate of occurrence, abnormal	mitfa^w2/w2; gjd2a^fh437/fh437	chemical treatment by environment: Mefloquine hydrochloride, chemical treatment by environment: pentetrazol	Figure 6 from Brunal et al., 2021
hindbrain neuronal action potential decreased rate of occurrence, abnormal	mitfa^w2/w2; gjd2a^fh437/fh437	chemical treatment by environment: Mefloquine hydrochloride, chemical treatment by environment: pentetrazol	Figure 6 from Brunal et al., 2021
inferior olive neuronal action potential increased rate of occurrence, ameliorated	mitfa^w2/w2; gjd2a^fh437/fh437	chemical treatment by environment: pentetrazol	Figure 1 from Brunal et al., 2021
ventral telencephalon neuronal action potential decreased rate of occurrence, abnormal	mitfa^w2/w2; gjd2a^fh437/fh437	chemical treatment by environment: Mefloquine hydrochloride, chemical treatment by environment: pentetrazol	Figure 6 from Brunal et al., 2021
rhombomere 7 neuronal action potential increased rate of occurrence, abnormal	mitfa^w2/w2; gjd2a^fh437/fh437	chemical treatment by environment: Mefloquine hydrochloride, chemical treatment by environment: pentetrazol	Figure 6 from Brunal et al., 2021
inferior olive neuronal action potential decreased rate of occurrence, abnormal	mitfa^w2/w2; gjd2a^fh437/fh437	chemical treatment by environment: Mefloquine hydrochloride	Figure 6 from Brunal et al., 2021
olfactory bulb neuronal action potential decreased rate of occurrence, abnormal	mitfa^w2/w2; gjd2a^fh437/fh437	chemical treatment by environment: Mefloquine hydrochloride	Figure 6 from Brunal et al., 2021
hypothalamus neuronal action potential increased rate of occurrence, exacerbated	mitfa^w2/w2; gjd2a^fh437/fh437	chemical treatment by environment: pentetrazol	Figure 1 from Brunal et al., 2021
ventral telencephalon neuronal action potential decreased rate of occurrence, abnormal	mitfa^w2/w2; gjd2a^fh437/fh437	chemical treatment by environment: Mefloquine hydrochloride	Figure 6 from Brunal et al., 2021
hindbrain neuronal action potential increased rate of occurrence, exacerbated	mitfa^w2/w2; gjd2a^fh437/fh437	chemical treatment by environment: pentetrazol	Figure 1 from Brunal et al., 2021
inferior olive neuronal action potential decreased rate of occurrence, abnormal	mitfa^w2/w2; gjd2a^fh437/fh437	chemical treatment by environment: Mefloquine hydrochloride, chemical treatment by environment: pentetrazol	Figure 6 from Brunal et al., 2021
telencephalon neuronal action potential decreased rate of occurrence, abnormal	mitfa^w2/w2; gjd2a^fh437/fh437	chemical treatment by environment: Mefloquine hydrochloride, chemical treatment by environment: pentetrazol	Figure 6 from Brunal et al., 2021
telencephalon neuronal action potential decreased rate of occurrence, abnormal	mitfa^w2/w2; gjd2a^fh437/fh437	chemical treatment by environment: Mefloquine hydrochloride	Figure 6 from Brunal et al., 2021
olfactory bulb neuronal action potential decreased rate of occurrence, abnormal	mitfa^w2/w2; gjd2a^fh437/fh437	chemical treatment by environment: Mefloquine hydrochloride, chemical treatment by environment: pentetrazol	Figure 6 from Brunal et al., 2021
brain ab3-gjd2 labeling decreased amount, abnormal	mitfa^w2/w2; gjd2a^fh437/fh437	standard conditions	Figure 3 from Brunal et al., 2021
vestibulospinal neuron neuronal action potential decreased rate of occurrence, abnormal	mitfa^w2/w2; gjd2a^fh437/fh437	control	Figure 1 from Brunal et al., 2021
habenula neuronal action potential decreased rate of occurrence, abnormal	mitfa^w2/w2; gjd2a^fh437/fh437	chemical treatment by environment: Mefloquine hydrochloride	Figure 6 from Brunal et al., 2021
reticulospinal neuron neuronal action potential decreased rate of occurrence, abnormal	mitfa^w2/w2; gjd2a^fh437/fh437	control	Figure 1 from Brunal et al., 2021
habenula neuronal action potential decreased rate of occurrence, abnormal	mitfa^w2/w2; gjd2a^fh437/fh437	chemical treatment by environment: Mefloquine hydrochloride, chemical treatment by environment: pentetrazol	Figure 6 from Brunal et al., 2021
ventral telencephalon neuronal action potential increased rate of occurrence, exacerbated	mitfa^w2/w2; gjd2a^fh437/fh437	chemical treatment by environment: pentetrazol	Figure 1 from Brunal et al., 2021
hindbrain neuronal action potential decreased rate of occurrence, abnormal	mitfa^w2/w2; gjd2a^fh437/fh437	chemical treatment by environment: Mefloquine hydrochloride	Figure 6 from Brunal et al., 2021

Citations