PUBLICATION
Astrocytes influence medulloblastoma phenotypes and CD133 surface expression
- Authors
- Gronseth, E., Gupta, A., Koceja, C., Kumar, S., Kutty, R.G., Rarick, K., Wang, L., Ramchandran, R.
- ID
- ZDB-PUB-201002-1
- Date
- 2020
- Source
- PLoS One 15: e0235852 (Journal)
- Registered Authors
- Ramchandran, Ramani
- Keywords
- none
- MeSH Terms
-
- AC133 Antigen/genetics*
- AC133 Antigen/metabolism
- Animals
- Astrocytes/metabolism*
- Cell Adhesion
- Cell Line, Tumor
- Cell Movement
- Cells, Cultured
- Culture Media, Conditioned
- Humans
- Medulloblastoma/metabolism*
- Neoplastic Stem Cells/drug effects
- Neoplastic Stem Cells/metabolism
- Neoplastic Stem Cells/physiology
- Nestin/genetics
- Nestin/metabolism
- Octamer Transcription Factor-3/genetics
- Octamer Transcription Factor-3/metabolism
- Phenotype*
- Tumor Microenvironment
- Zebrafish
- PubMed
- 32628717 Full text @ PLoS One
Citation
Gronseth, E., Gupta, A., Koceja, C., Kumar, S., Kutty, R.G., Rarick, K., Wang, L., Ramchandran, R. (2020) Astrocytes influence medulloblastoma phenotypes and CD133 surface expression. PLoS One. 15:e0235852.
Abstract
The medulloblastoma (MB) microenvironment is diverse, and cell-cell interactions within this milieu is of prime importance. Astrocytes, a major component of the microenvironment, have been shown to impact primary tumor cell phenotypes and metastasis. Based on proximity of MB cells and astrocytes in the brain microenvironment, we investigated whether astrocytes may influence MB cell phenotypes directly. Astrocyte conditioned media (ACM) increased Daoy MB cell invasion, adhesion, and in vivo cellular protrusion formation. ACM conditioning of MB cells also increased CD133 surface expression, a key cancer stem cell marker of MB. Additional neural stem cell markers, Nestin and Oct-4A, were also increased by ACM conditioning, as well as neurosphere formation. By knocking down CD133 using short interfering RNA (siRNA), we showed that ACM upregulated CD133 expression in MB plays an important role in invasion, adhesion and neurosphere formation. Collectively, our data suggests that astrocytes influence MB cell phenotypes by regulating CD133 expression, a key protein with defined roles in MB tumorgenicity and survival.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping