PUBLICATION
A cis-regulatory module upstream of deltaC regulated by Ntla and Tbx16 drives expression in the tailbud, presomitic mesoderm and somites
- Authors
- Jahangiri, L., Nelson, A.C., Wardle, F.C.
- ID
- ZDB-PUB-150817-2
- Date
- 2012
- Source
- Developmental Biology 371: 110-120 (Journal)
- Registered Authors
- Nelson, Andrew, Wardle, Fiona
- Keywords
- CRM; Ntla; Tbx16; T-box transcription factors; deltaC
- MeSH Terms
-
- Animals
- Fetal Proteins
- Gene Expression Regulation, Developmental/physiology*
- Mutagenesis, Site-Directed
- Intracellular Signaling Peptides and Proteins/genetics
- Polymerase Chain Reaction
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/pharmacology*
- Molecular Sequence Data
- Sequence Analysis, DNA
- Membrane Proteins/genetics
- DNA Primers/genetics
- Tail/embryology
- Tail/metabolism*
- Somites/embryology
- Somites/metabolism*
- Base Sequence
- Chromatin Immunoprecipitation
- Mesoderm/embryology
- Mesoderm/metabolism*
- T-Box Domain Proteins/genetics
- T-Box Domain Proteins/pharmacology*
- Electrophoretic Mobility Shift Assay
- PubMed
- 22877946 Full text @ Dev. Biol.
Citation
Jahangiri, L., Nelson, A.C., Wardle, F.C. (2012) A cis-regulatory module upstream of deltaC regulated by Ntla and Tbx16 drives expression in the tailbud, presomitic mesoderm and somites. Developmental Biology. 371:110-120.
Abstract
Somites form by an iterative process from unsegmented, presomitic mesoderm (PSM). Notch pathway components, such as deltaC (dlc) have been shown to play a role in this process, while the T-box transcription factors Ntla and Tbx16 regulate somite formation upstream of this by controlling supply and movement of cells into the PSM during gastrulation and tailbud outgrowth. In this work, we report that Ntla and Tbx16 play a more explicit role in segmentation by directly regulating dlc expression. In addition we describe a cis-regulatory module (CRM) upstream of dlc that drives expression of a reporter in the tailbud, PSM and somites during somitogenesis. This CRM is bound by both Ntla and Tbx16 at a cluster of T-box binding sites, which are required in combination for activation of the CRM.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping