PUBLICATION
Vesicular stomatitis virus enables gene transfer and transsynaptic tracing in a wide range of organisms
- Authors
- Mundell, N.A., Beier, K.T., Pan, Y.A., Lapan, S.W., Göz Aytürk, D., Berezovskii, V.K., Wark, A.R., Drokhlyansky, E., Bielecki, J., Born, R.T., Schier, A.F., Cepko, C.L.
- ID
- ZDB-PUB-150218-3
- Date
- 2015
- Source
- The Journal of comparative neurology 523(11): 1639-63 (Journal)
- Registered Authors
- Pan, Y. Albert, Schier, Alexander
- Keywords
- AB_10562207, AB_531908, AB_591819, RRID: AB_10053281, SciRes_000161, VSV, anterograde, centrifugal, in vivo, polysynaptic, retina, retrograde, transsynaptic, visual pathways
- MeSH Terms
-
- Animals
- Cell Line/cytology
- Cell Line/metabolism
- Gene Transfer Techniques*
- Glycoproteins/genetics
- Glycoproteins/metabolism
- Humans
- Invertebrates/anatomy & histology
- Invertebrates/metabolism
- Neuroanatomical Tract-Tracing Techniques*
- Neurons/cytology
- Neurons/metabolism
- Rabies virus/genetics
- Vertebrates/anatomy & histology
- Vertebrates/metabolism
- Vesicular Stomatitis*
- Vesiculovirus/genetics*
- Viral Proteins/genetics
- Viral Proteins/metabolism
- Visual Pathways/anatomy & histology
- Visual Pathways/metabolism
- PubMed
- 25688551 Full text @ J. Comp. Neurol.
Citation
Mundell, N.A., Beier, K.T., Pan, Y.A., Lapan, S.W., Göz Aytürk, D., Berezovskii, V.K., Wark, A.R., Drokhlyansky, E., Bielecki, J., Born, R.T., Schier, A.F., Cepko, C.L. (2015) Vesicular stomatitis virus enables gene transfer and transsynaptic tracing in a wide range of organisms. The Journal of comparative neurology. 523(11):1639-63.
Abstract
Current limitations in technology have prevented an extensive analysis of the connections among neurons, particularly within non-mammalian organisms. We developed a transsynaptic viral tracer originally for use in mice, and then tested its utility in a broader range of organisms. By engineering the vesicular stomatitis virus (VSV) to encode a fluorophore and either the rabies virus glycoprotein (RABV-G) or its own glycoprotein (VSV-G), we created viruses that can transsynaptically label neuronal circuits in either the retrograde or anterograde direction, respectively. The vectors were investigated for their utility as polysynaptic tracers of chicken and zebrafish visual pathways. They showed patterns of connectivity consistent with previously characterized visual system connections, and revealed several potentially novel connections. Further, these vectors were shown to infect neurons in several other vertebrates, including Old and New World monkeys, seahorses, axolotls, and Xenopus. They were also shown to infect two invertebrates, Drosophila melanogaster, and the box jellyfish, Tripedalia cystophora, a species previously intractable for gene transfer, though no clear evidence of transsynaptic spread was observed in these species. These vectors provide a starting point for transsynaptic tracing in most vertebrates, and are also excellent candidates for gene transfer in organisms that have been refractory to other methods. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping