PUBLICATION

A Pak1/Erk Signaling Module Acts through Gata6 to Regulate Cardiovascular Development in Zebrafish

Authors
Kelly, M.L., Astsaturov, A., Rhodes, J., Chernoff, J.
ID
ZDB-PUB-140516-11
Date
2014
Source
Developmental Cell   29: 350-9 (Journal)
Registered Authors
Rhodes, Jennifer
Keywords
none
MeSH Terms
  • Animals
  • Cardiovascular Abnormalities/genetics
  • Cardiovascular System/embryology*
  • Cell Movement
  • Extracellular Signal-Regulated MAP Kinases/metabolism*
  • GATA Transcription Factors/metabolism*
  • Gene Expression Regulation, Developmental
  • Gene Knockdown Techniques
  • Heart/embryology
  • MAP Kinase Kinase 1/metabolism
  • Morpholinos/genetics
  • Neural Crest/embryology*
  • Phosphorylation
  • Signal Transduction/genetics
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Zebrafish Proteins/metabolism*
  • p21-Activated Kinases/genetics
  • p21-Activated Kinases/metabolism*
PubMed
24823378 Full text @ Dev. Cell
Abstract
Proper neural crest development and migration is critical during embryonic development, but the molecular mechanisms regulating this process remain incompletely understood. Here, we show that the protein kinase Erk, which plays a central role in a number of key developmental processes in vertebrates, is regulated in the developing neural crest by p21-activated protein kinase 1 (Pak1). Furthermore, we show that activated Erk signals by phosphorylating the transcription factor Gata6 on a conserved serine residue to promote neural crest migration and proper formation of craniofacial structures, pigment cells, and the outflow tract of the heart. Our data suggest an essential role for Pak1 as an Erk activator, and Gata6 as an Erk target, during neural crest development.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping