PUBLICATION
Schwann cell spectrins modulate peripheral nerve myelination
- Authors
- Susuki, K., Raphael, A.R., Ogawa, Y., Stankewich, M.C., Peles, E., Talbot, W.S., and Rasband, M.N.
- ID
- ZDB-PUB-110518-38
- Date
- 2011
- Source
- Proceedings of the National Academy of Sciences of the United States of America 108(19): 8009-8014 (Journal)
- Registered Authors
- Raphael, Alya, Talbot, William S.
- Keywords
- none
- MeSH Terms
-
- Actins/antagonists & inhibitors
- Actins/physiology
- Animals
- Base Sequence
- Cell Polarity
- Cytoskeleton/physiology
- Gene Knockdown Techniques
- Mutation
- Myelin Sheath/physiology*
- Peripheral Nerves/physiology*
- RNA Interference
- Rats
- Rats, Sprague-Dawley
- Schwann Cells/cytology
- Schwann Cells/physiology*
- Sciatic Nerve/cytology
- Sciatic Nerve/injuries
- Sciatic Nerve/physiology
- Spectrin/antagonists & inhibitors
- Spectrin/deficiency
- Spectrin/genetics
- Spectrin/physiology*
- Zebrafish/genetics
- Zebrafish/physiology
- Zebrafish Proteins/deficiency
- Zebrafish Proteins/genetics
- Zebrafish Proteins/physiology
- PubMed
- 21518878 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Susuki, K., Raphael, A.R., Ogawa, Y., Stankewich, M.C., Peles, E., Talbot, W.S., and Rasband, M.N. (2011) Schwann cell spectrins modulate peripheral nerve myelination. Proceedings of the National Academy of Sciences of the United States of America. 108(19):8009-8014.
Abstract
During peripheral nerve development, Schwann cells ensheathe axons and form myelin to enable rapid and efficient action potential propagation. Although myelination requires profound changes in Schwann cell shape, how neuron-glia interactions converge on the Schwann cell cytoskeleton to induce these changes is unknown. Here, we demonstrate that the submembranous cytoskeletal proteins αII and βII spectrin are polarized in Schwann cells and colocalize with signaling molecules known to modulate myelination in vitro. Silencing expression of these spectrins inhibited myelination in vitro, and remyelination in vivo. Furthermore, myelination was disrupted in motor nerves of zebrafish lacking αII spectrin. Finally, we demonstrate that loss of spectrin significantly reduces both F-actin in the Schwann cell cytoskeleton and the Nectin-like protein, Necl4, at the contact site between Schwann cells and axons. Therefore, we propose αII and βII spectrin in Schwann cells integrate the neuron-glia interactions mediated by membrane proteins into the actin-dependent cytoskeletal rearrangements necessary for myelination.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping