PUBLICATION
The miR-143-adducin3 pathway is essential for cardiac chamber morphogenesis
- Authors
- Deacon, D.C., Nevis, K.R., Cashman, T.J., Zhou, Y., Zhao, L., Washko, D., Guner-Ataman, B., Burns, C.G., and Burns, C.E.
- ID
- ZDB-PUB-100518-4
- Date
- 2010
- Source
- Development (Cambridge, England) 137(11): 1887-1896 (Journal)
- Registered Authors
- Burns (Erter), Caroline, Burns, Geoff, Cashman, Tim, Deacon, Dekker, Zhao, Long, Zhou, Yong
- Keywords
- microRNA, miR-143(mir143), Adducin, Zebrafish, Heart development, Organogenesis
- MeSH Terms
-
- 3' Untranslated Regions
- Actins/metabolism
- Animals
- Base Sequence
- Calmodulin-Binding Proteins/genetics*
- Calmodulin-Binding Proteins/physiology
- Gene Expression Regulation, Developmental
- Heart/embryology*
- In Situ Hybridization
- MicroRNAs/genetics*
- Myocytes, Cardiac/cytology
- Myocytes, Cardiac/physiology
- Oligodeoxyribonucleotides, Antisense/genetics
- Sequence Homology, Nucleic Acid
- Zebrafish/embryology*
- Zebrafish/genetics*
- Zebrafish/physiology
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/physiology
- PubMed
- 20460367 Full text @ Development
Citation
Deacon, D.C., Nevis, K.R., Cashman, T.J., Zhou, Y., Zhao, L., Washko, D., Guner-Ataman, B., Burns, C.G., and Burns, C.E. (2010) The miR-143-adducin3 pathway is essential for cardiac chamber morphogenesis. Development (Cambridge, England). 137(11):1887-1896.
Abstract
Discovering the genetic and cellular mechanisms that drive cardiac morphogenesis remains a fundamental goal, as three-dimensional architecture greatly impacts functional capacity. During development, accurately contoured chambers balloon from a primitive tube in a process characterized by regional changes in myocardial cell size and shape. How these localized changes are achieved remains elusive. Here, we show in zebrafish that microRNA-143 (miR-143) is required for chamber morphogenesis through direct repression of adducin3 (add3), which encodes an F-actin capping protein. Knockdown of miR-143 or disruption of the miR-143-add3 interaction inhibits ventricular cardiomyocyte F-actin remodeling, which blocks their normal growth and elongation and leads to ventricular collapse and decreased contractility. Using mosaic analyses, we find that miR-143 and add3 act cell-autonomously to control F-actin dynamics and cell morphology. As proper chamber emergence relies on precise control of cytoskeletal polymerization, Add3 represents an attractive target to be fine-tuned by both uniform signals, such as miR-143, and undiscovered localized signals. Together, our data uncover the miR-143-add3 genetic pathway as essential for cardiac chamber formation and function through active adjustment of myocardial cell morphology.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping