PUBLICATION
Zebrafish cdx1b regulates differentiation of various intestinal cell lineages
- Authors
- Chen, Y.H., Lu, Y.F., Ko, T.Y., Tsai, M.Y., Lin, C.Y., Lin, C.C., and Hwang, S.P.
- ID
- ZDB-PUB-090309-11
- Date
- 2009
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 238(5): 1021-1032 (Journal)
- Registered Authors
- Hwang, Sheng-Ping L.
- Keywords
- intestine, cdx1b, embryos
- MeSH Terms
-
- Animals
- Cell Differentiation*/genetics
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/metabolism*
- Embryonic Development/genetics
- Embryonic Development/physiology
- Enterocytes/cytology*
- Enterocytes/metabolism
- Enterocytes/ultrastructure
- Gene Expression Regulation, Developmental/genetics
- Gene Expression Regulation, Developmental/physiology
- Gene Knockdown Techniques
- Goblet Cells/cytology*
- Goblet Cells/metabolism
- Goblet Cells/ultrastructure
- Homeodomain Proteins/genetics
- Homeodomain Proteins/metabolism*
- Intestines/cytology
- Intestines/embryology
- Intestines/metabolism*
- Intestines/ultrastructure
- Microscopy, Electron, Transmission
- Oligonucleotides, Antisense/genetics
- Oligonucleotides, Antisense/metabolism
- Symporters/metabolism*
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 19253392 Full text @ Dev. Dyn.
Citation
Chen, Y.H., Lu, Y.F., Ko, T.Y., Tsai, M.Y., Lin, C.Y., Lin, C.C., and Hwang, S.P. (2009) Zebrafish cdx1b regulates differentiation of various intestinal cell lineages. Developmental Dynamics : an official publication of the American Association of Anatomists. 238(5):1021-1032.
Abstract
Both antisense morpholino oligonucleotide (MO)-mediated knockdown and overexpression experiments were performed to analyze zebrafish cdx1b's function in intestinal cell differentiation. Substantial reductions in goblet cell numbers were detected in intestines of 102- and 120-hours post-fertilization (hpf) cdx1b MO-injected embryos (morphants) compared to cdx1b-4-base mismatched (4mm)-MO-injected and wild type embryos. A significant decrease in enteroendocrine cell numbers was also observed in intestines of 96-hpf cdx1b morphants. Furthermore, ectopic cdx1b expression caused notable increases in respective cell numbers of enteroendocrine and goblet cells in intestines of 96- and 98-hpf injected embryos. Decreased PepT1 expression was detected in enterocytes of intestines in cdx1b morphants from 80 to 102 hr of development. In addition, increased cell proliferation was detected in intestines of cdx1b morphants. Overall, our results suggest that zebrafish cdx1b plays important roles in regulating intestinal cell proliferation and the differentiation of various intestinal cell lineages.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping