PUBLICATION
HNF factors form a network to regulate liver-enriched genes in zebrafish
- Authors
- Cheng, W., Guo, L., Zhang, Z., Soo, H.M., Wen, C., Wu, W., and Peng, J.
- ID
- ZDB-PUB-060501-4
- Date
- 2006
- Source
- Developmental Biology 294(2): 482-496 (Journal)
- Registered Authors
- Peng, Jinrong
- Keywords
- Microarray, Liver-enriched genes, HNF factors, Liver development, Promoter analysis, Functional genomics
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Embryo, Nonmammalian/anatomy & histology
- Embryo, Nonmammalian/physiology
- Gene Expression Profiling
- Gene Expression Regulation, Developmental*
- Hepatocyte Nuclear Factors/genetics
- Hepatocyte Nuclear Factors/metabolism*
- Humans
- In Situ Hybridization
- Liver/embryology
- Liver/physiology*
- Molecular Sequence Data
- Oligonucleotide Array Sequence Analysis
- Oligonucleotides, Antisense/genetics
- Oligonucleotides, Antisense/metabolism
- Promoter Regions, Genetic
- Sequence Alignment
- Zebrafish/anatomy & histology
- Zebrafish/embryology*
- Zebrafish/genetics*
- Zebrafish/physiology
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 16631158 Full text @ Dev. Biol.
Citation
Cheng, W., Guo, L., Zhang, Z., Soo, H.M., Wen, C., Wu, W., and Peng, J. (2006) HNF factors form a network to regulate liver-enriched genes in zebrafish. Developmental Biology. 294(2):482-496.
Abstract
Defects in some of liver-enriched genes in mammals will cause liver- and/or blood-related diseases. However, due to the fact that embryogenesis happens intrauterinally in the mammals, the function of these liver-enriched genes during liver organogenesis is poorly studied. We report here the identification of 129 genuine liver-enriched genes in adult zebrafish and show that, through in situ hybridization, 69 of these genes are also enriched in the embryonic liver. External embryogenesis coupled with the well-established morpholino-mediated gene knock-down technique in zebrafish offers us a unique opportunity to study if this group of genes plays any role during liver organogenesis in the future. As an example, preliminary study using morpholino-mediated gene knock-down method revealed that a novel liver-enriched gene leg1 is crucial for the liver expansion growth. We also report the analysis of promoter regions of 51 liver-enriched genes by searching putative binding sites for Hnf1, Hnf3, Hnf4 and Hnf6, four key transcription factors enriched in the liver. We found that promoter regions of majority of liver-enriched genes contain putative binding sites for more than one HNF factors, suggesting that most of liver-enriched genes are likely co-regulated by different combination of HNF factors. This observation supports the hypothesis that these four liver-enriched transcription factors form a network in controlling the expression of liver-specific or -enriched genes in the liver.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping