PUBLICATION
Separate Na,K-ATPase genes are required for otolith formation and semicircular canal development in zebrafish
- Authors
- Blasiole, B., Canfield, V.A., Vollrath, M.A., Huss, D., Mohideen, M.A., Dickman, J.D., Cheng, K.C., Fekete, D.M., and Levenson, R.
- ID
- ZDB-PUB-060403-5
- Date
- 2006
- Source
- Developmental Biology 294(1): 148-160 (Journal)
- Registered Authors
- Blasiole, Brian, Canfield, Victor, Cheng, Keith C., Fekete, Donna Marie, Levenson, Robert, Mohideen, Manzoor Pallithotangal
- Keywords
- Zebrafish, Na,K-ATPase, Sodium pump, Morpholino, Inner ear, Otolith, Semicircular canal, mRNA rescue
- MeSH Terms
-
- Animals
- Ear/growth & development
- Embryo, Nonmammalian
- Isoenzymes
- Morphogenesis
- Otolithic Membrane/enzymology*
- Protein Subunits
- Rats
- Semicircular Canals/enzymology*
- Sodium-Potassium-Exchanging ATPase/genetics*
- Sodium-Potassium-Exchanging ATPase/physiology
- Zebrafish
- PubMed
- 16566913 Full text @ Dev. Biol.
Citation
Blasiole, B., Canfield, V.A., Vollrath, M.A., Huss, D., Mohideen, M.A., Dickman, J.D., Cheng, K.C., Fekete, D.M., and Levenson, R. (2006) Separate Na,K-ATPase genes are required for otolith formation and semicircular canal development in zebrafish. Developmental Biology. 294(1):148-160.
Abstract
We have investigated the role of Na,K-ATPase genes in zebrafish ear development. Six Na,K-ATPase genes are differentially expressed in the developing zebrafish inner ear. Antisense morpholino knockdown of Na,K-ATPase alpha1a.1 expression blocked formation of otoliths. This effect was phenocopied by treatment of embryos with ouabain, an inhibitor of Na,K-ATPase activity. The otolith defect produced by morpholinos was rescued by microinjection of zebrafish alpha1a.1 or rat alpha1 mRNA, while the ouabain-induced defect was rescued by expression of ouabain-resistant zebrafish alpha1a.1 or rat alpha1 mRNA. Knockdown of a second zebrafish alpha subunit, alpha1a.2, disrupted development of the semicircular canals. Knockdown of Na,K-ATPase beta2b expression also caused an otolith defect, suggesting that the beta2b subunit partners with the alpha1a.1 subunit to form a Na,K-ATPase required for otolith formation. These results reveal novel roles for Na,K-ATPase genes in vestibular system development and indicate that different isoforms play distinct functional roles in formation of inner ear structures. Our results highlight zebrafish gene knockdown-mRNA rescue as an approach that can be used to dissect the functional properties of zebrafish and mammalian Na,K-ATPase genes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping