PUBLICATION
The zebrafish bcl-2 homologue Nrz controls development during somitogenesis and gastrulation via apoptosis-dependent and -independent mechanisms
- Authors
- Arnaud, E., Ferri, K.F., Thibaut, J., Haftek-Terreau, Z., Aouacheria, A., Le Guellec, D., Lorca, T., and Gillet, G.
- ID
- ZDB-PUB-051114-13
- Date
- 2006
- Source
- Cell death and differentiation 13(7): 1128-1137 (Journal)
- Registered Authors
- Gillet, Germain, Le Guellec, Dominique
- Keywords
- apoptosis, Bcl-2, gastrulation, Nr-13, somites, zebrafish
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Apoptosis/physiology*
- Blotting, Western
- COS Cells
- Chlorocebus aethiops
- Cloning, Molecular
- Gastrula/cytology
- Gastrula/metabolism
- Gastrula/physiology*
- Gene Expression Regulation, Developmental
- In Situ Hybridization
- Microscopy, Confocal
- Molecular Sequence Data
- Oligonucleotides, Antisense/genetics
- Proto-Oncogene Proteins/genetics
- Proto-Oncogene Proteins/metabolism
- Proto-Oncogene Proteins/physiology*
- Proto-Oncogene Proteins c-bcl-2/genetics
- Proto-Oncogene Proteins c-bcl-2/metabolism
- Proto-Oncogene Proteins c-bcl-2/physiology
- Reverse Transcriptase Polymerase Chain Reaction
- Sequence Homology, Amino Acid
- Somites/cytology
- Somites/metabolism
- Somites/physiology*
- Transcription Factors/genetics
- Transcription Factors/metabolism
- Transcription Factors/physiology
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/physiology
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Zebrafish Proteins/physiology*
- PubMed
- 16282981 Full text @ Cell Death Differ.
Citation
Arnaud, E., Ferri, K.F., Thibaut, J., Haftek-Terreau, Z., Aouacheria, A., Le Guellec, D., Lorca, T., and Gillet, G. (2006) The zebrafish bcl-2 homologue Nrz controls development during somitogenesis and gastrulation via apoptosis-dependent and -independent mechanisms. Cell death and differentiation. 13(7):1128-1137.
Abstract
Although the role of the b-cell lymphoma (Bcl)-2 family of apoptosis inhibitors is well documented in tumor cells and tissue morphogenesis, their role during the early development of vertebrates is unknown. Here, we characterize Nrz, a new Bcl-2-related inhibitor of apoptosis in zebrafish. Nrz is a mitochondrial protein, antagonizing the death-accelerator Bax. The nrz gene is mainly expressed during gastrulation and somitogenesis. The knockdown of nrz with antisense morpholinos leads to alterations of the somites, correlated with an increase in apoptosis. In addition, earlier during development, in the zebrafish gastrula, nrz knockdown results in an increase of snail-1 expression at the margin and frequent gastrulation arrest at the shield stage, independently of apoptosis. Together these data suggest that Nrz, in addition to its effect on apoptosis, contributes to cell movements during gastrulation by negatively regulating the expression of Snail-1, a transcription factor that controls cell adhesion.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping