Person
Gillet, Germain
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Biography and Research Interest
Current position
Professor (PU-PH) Faculty of medicine, Université Claude Bernard Lyon 1 (UCBL) Group leader at CRCL
Current research activity
The molecular mechanisms governing tumor progression, including metastasis development, are still poorly understood. We showed that some members of the Bcl-2 family are able to control cell migration in a Ca2+-dependent manner via their direct interactions with Ca2+ channels, independently of their involvement in apoptosis. Using genetically engineered mouse and zebrafish models, we are currently analyzing the molecular mechanisms by which members of the Bcl-2 family influence cell survival and migration. We intend to (i) identify the signaling networks that lead to the modulation of Ca2+ homeostasis and cell movements by Bcl-2 family proteins (ii) identify the factors involved in apoptosis progression and cell migration (iii) review the roles played by these factors, depending on the physiopathological context.
It is expected that this work will lead to the identification of novel prognosis markers and deliver potential molecular targets for inhibiting tumor growth and metastases onset.
Education & Degrees
Ecole normale supérieure (biochemistry, organic chemistry, Paris, 1978-84)
Agrégation biochimie-génie biologique (Paris, 1983)
PhD, Institut Pasteur (Moleculer pharmacology of serotonin receptors, Paris, 1985)
Habilitation, UCBL (Control of cell cycle & cell death by the v-src oncogene, Lyon,1996)
Scientific career
2011-present : Group leader, Centre de Recherche en Cancérologie de Lyon
1997-2010 : Group leader, Institut de Biologie et Chimie des Protéines, Lyon
1988-97 : Research associate, Ecole normale supérieure de Lyon
1985-87 : Post doctoral fellow, Institut Curie, Paris
1983-84 : PhD student, Institut Pasteur, Paris
Professor (PU-PH) Faculty of medicine, Université Claude Bernard Lyon 1 (UCBL) Group leader at CRCL
Current research activity
The molecular mechanisms governing tumor progression, including metastasis development, are still poorly understood. We showed that some members of the Bcl-2 family are able to control cell migration in a Ca2+-dependent manner via their direct interactions with Ca2+ channels, independently of their involvement in apoptosis. Using genetically engineered mouse and zebrafish models, we are currently analyzing the molecular mechanisms by which members of the Bcl-2 family influence cell survival and migration. We intend to (i) identify the signaling networks that lead to the modulation of Ca2+ homeostasis and cell movements by Bcl-2 family proteins (ii) identify the factors involved in apoptosis progression and cell migration (iii) review the roles played by these factors, depending on the physiopathological context.
It is expected that this work will lead to the identification of novel prognosis markers and deliver potential molecular targets for inhibiting tumor growth and metastases onset.
Education & Degrees
Ecole normale supérieure (biochemistry, organic chemistry, Paris, 1978-84)
Agrégation biochimie-génie biologique (Paris, 1983)
PhD, Institut Pasteur (Moleculer pharmacology of serotonin receptors, Paris, 1985)
Habilitation, UCBL (Control of cell cycle & cell death by the v-src oncogene, Lyon,1996)
Scientific career
2011-present : Group leader, Centre de Recherche en Cancérologie de Lyon
1997-2010 : Group leader, Institut de Biologie et Chimie des Protéines, Lyon
1988-97 : Research associate, Ecole normale supérieure de Lyon
1985-87 : Post doctoral fellow, Institut Curie, Paris
1983-84 : PhD student, Institut Pasteur, Paris
Non-Zebrafish Publications
- D. MANSUY, P. BATTIONI, J.P. MAHY and G. GILLET
Comparison of the hemoglobin reactions with methyl and phenylhydrazine: intermediate formation of a hemoglobin Fe(II)-methyldiazene complex
Biochem. Biophys. Res. Com. (1982), 106, 30-6
- P. BATTIONI, J.P. MAHY, G. GILLET and D. MANSUY
Iron porphyrin dependent oxidation of methyl and phenylhydrazine
J. Am. Chem. Soc. (1983), 105, 1399-401
- J.C. ROUSSELLE, G. GILLET and G. FILLION
Solubilization and characterization of 3H-5HT high affinity binding sites
J. Pharmacol. (1984), 15, 421-38
- C. ROBAUT, M.P. FILLION, S. DUFOIS, C. FAYOLLE, J.C. ROUSSELLE, G. GILLET, S. BENKIRANE and G. FILLION
Multiple high affinity binding sites for 5-Hydroxytryptamine: a new class of sites distinct from 5HT1 and S2
Brain Res. (1985), 346, 250-62
- G. GILLET, S. AMMOR and G. FILLION
Serotonin inhibits acetylcholine release from rat striatum slices: evidence for a presynaptic receptor-mediated effect
J. Neurochem. (1985), 45, 1687-91
- D. MICHEL, G. GILLET, M. VOLOVITCH, B. PESSAC, G. CALOTHY and G. BRUN
Expression of a novel gene encoding a 52 Kd precursor protein is induced by different retroviral oncogenes in quail neuroretinal cells
Oncogene Res. (1989), 4, 127-36
- M. GUERMAH, G. GILLET, D. MICHEL, D. LAUGIER, G. BRUN and G. CALOTHY
Down-regulation by pp60v-src of genes specifically expressed and developmentally regulated in post-mitotic quail neuroretina cells.
Mol. Cell. Biol. (1990), 10, 3584-90
- G. GILLET, D. MICHEL, P. CRISANTI, M. GUERIN, Y. HERAULT, B. PESSAC, G. CALOTHY, G. BRUN and M. VOLOVITCH
Serum factors and v-src control two complementary mitogenic pathways in quail neuroretina cells in culture
Oncogene (1993), 8, 565-74
- G. GILLET, M. GUERIN , A. TREMBLEAU and G. BRUN
A Bcl-2-related gene is activated in avian cells transformed by the Rous sarcoma virus
EMBO J. (1995), 14, 1372-81
- S. NORTH, X. ESPANEL, B. TAVITIAN, G. BRUN and G. GILLET
Two distinct regulatory elements control quail cdc2 transcription. Possible involvement in the control of retinoblasts differentiation
Cell Growth & Differ. (1996), 7, 339-49
- M. MANGENEY, J. R. SCHMITT, Y. LEVERRIER, J.THOMAS, J. MARVEL, G. BRUN and G. GILLET
The product of the v-src-inducible gene nr-13, is a potent antiapoptotic factor
Oncogene (1996), 13, 1441-46
- X. ESPANEL, A. KASTNER, O. STETTLER, B. TAVITIAN, G. BRUN and G. GILLET
p34cdc2 and mitotic cyclins expression in the developing quail neuroretina
Int. J. Dev. Biol. (1997), 41, 469-76
- R.M. LEE, G. GILLET and P. NEIMAN
Molecular event in avian neoplasia: regulators of cell death in development of B-cell lymphomas in the chicken bursa of Fabricius
Avian Pathol. (1998), 27, S16-S20
- X. ESPANEL, L. LE CAM, S. NORTH, C. SARDET, G. BRUN and G. GILLET
Regulation of E2F-1 gene expression in the developing quail neuroretina
Oncogene (1998), 17, 585-94
- S. NORTH, F. BANTIGNIES, X. ESPANEL, B. VIOLLET, P. JALINOT, G. BRUN and G. GILLET
Regulation of cdc2 gene expression by the upstream stimulatory factors (USFs)
Oncogene (1999), 18, 1945-55
- R.M. LEE, G. GILLET , J BURNSIDE, S.J. THOMAS and P. NEIMAN
The role of Nr-13 in regulation of programmed cell death in the bursa of Fabricius
Genes & Dev. (1999) 13, 718-28
- A. AOUACHERIA, E. ARNAUD, S. VENET, P. LALLE, M. GOUY, D. RIGAL and G. GILLET
Nrh, a human homologue of Nr-13 associates with Bcl-Xs and is an inhibitor of apoptosis
Oncogene (2001), 20, 5846-55
- A. AOUACHERIA, S. ORY, J.R. SCHMITT, D. RIGAL, P. JURDIC and G. GILLET
p60v-src and serum control cell shape and apoptosis via distinct pathways in quail neuroretina cells
Oncogene (2002) 21, 1171-86
- M. MORADI-AMÉLI, T. LORCA, D. FICHEUX, A. DI PIETRO and G. GILLET
Interaction between the anti-apoptotic protein Nr-13 and cytochrome c. Antagonistic effect of the BH3 domain of Bax
Biochemistry (2002) 41, 8540-50
- P. LALLE, A. AOUACHERIA, A. DUMONT-MISCOPEIN, C. GEOURJON, M. JAMBON, S. VENET, H. BOBICHON, P. COLAS, G. DELEAGE and G. GILLET
Electrostatic Interactions Between Bcl-2 Homology Domains BH3 and BH4 are Essential for Nr–13 Anti-Apoptotic Activity
Biochem. J. (2002) 368, 213-21
- A AOUACHERIA, B. NÉEL, Z. BOUAZIZ, D. RIGAL, N. WALCHSHOFER, J. PARIS, H. FILLION and G. GILLET
Carbazolequinones induce caspase-dependent cell death in quail neuroretina cells transformed by RSV.
Biochem. Pharmacol. (2002) 64, 1605-16
- A. AOUACHERIA, M. BANYAI, D. RIGAL, C.J. SCHMIDT and G. GILLET
Characterization of vnr-13, the first alphaherpesvirus gene of the bcl-2 family.
Virology (2003) 316, 256-66
- A. GIRARD-EGROT, JP. CHAUVET, G. GILLET* , and M. AMELI-MORADI*,
The BH3 domain of Bax prevents the insertion of the Nr-13 survival protein into phospholipid monolayers
J. Mol. Biol. (2004) 335, 321-31
- J. C. M. MOMBACH, D. ROBERT, F. GRANER, G. GILLET, G. L. THOMAS, M. IDIART and JP. RIEU
Rounding of aggregates of biological cells: Experiments and Simulations
Physica A (2005) 352, 525-34
- B. NEEL, A. AOUACHERIA, AL. NOUVION and G. GILLET
Distinct protease pathways control cell shape and apoptosis in v-src-transformed quail neuroretina cells
Exp. Cell Res. (2005) 311, 106-16
- NOUVION AL, THIBAUT J, LOHEZ O, VENET S , AMELI-MORADI M, GILLET G.* and LALLE P.
Modulation of the anti-apoptotic protein Nr13 activity by peptide aptamers
Oncogene (2007) 26, 701-10
- BD. NÉEL, J. LOPEZ, A. CHABADEL and G. GILLET
Lithium suppresses motility and invasivity of v-src-transformed cells via glutathione-dependent activation of phosphotyrosine phosphatases
Oncogene (2009) 28, 3246–60
- Y. GUILLEMIN, P. LALLE, G. GILLET, JF GUERIN, S. HAMAMAH and A. AOUACHERIA
Oocytes and early embryos selectively express the survival factor BCL2L10
J. Mol. Med. (2009) 87 : 923-40
- B. M. MALIKOWSKI, B. D NEEL, Y. WU, L. WANG, N. A RODRIGUES, G. GILLET and P. A. BEDARD
Cellular processes of v-Src transformation revealed by gene profiling of primary cells - implications for human cancer
BMC Cancer (2010) 10 : 41 doi: 10.1186/1471-2407-10-41 «highly accessed »
- J.G. VALERO, L. SANCEY, J. KUCHARCZAK, Y. GUILLEMIN, D. GIMENEZ, J. PRUDENT, G. GILLET, J. SALGADO, J.L. COLL, and A. AOUACHERIA
Bax-derived membrane active peptides act as potent and direct inducers of apoptosis in cancer cells
J. Cell Sci. (2010) 124 : 556-64
- C. HESLING, L. FATTET, G. TEYRE, D. JURY, C. VANBELLE, A. PUISIEUX, A.P. MOREL, G. GILLET, I. MIKAELIAN and R. RIMOKH
Antagonistic regulation of EMT by TIF1γ and Smad4 in mammary epithelial cells
EMBO Rep. (2011) 12: 665 -72
- Y. GUILLEMIN, A. CORNUT-THIBAUT, F. PENIN, G. GILLET and A. AOUACHERIA
Characterization of unique signature sequences in the divergent maternal protein BCL2L10
Mol. Biol. Evol. (2011) 28 : 3271-83
- J. THIBAUT, Y. MÉRIEUX, D. RIGAL and G. GILLET
A novel assay for the detection of anti-human platelet antigen antibodies (HPA-1a) based on the peptide aptamer technology
Haematologica (2011) 97:696-704
- J. LOPEZ, C. HESLING, JULIEN PRUDENT, N. POPGEORGIEV, R. GADET, I. MIKAELIAN, R.RIMOKH, G.GILLET* and P. GONZALO*
Src induces resistance to apoptosis of tumor cells by activating the ERK1/2 dependent phosphorylation of Bik and its further proteasomal degradation
Cell Death & Differ. (2012) 9:1459-69
- J.G. VALERO, A. CORNUT-THIBAUT, R. JUGE, A.L. DEBAUD, D. GIMENEZ, G. GILLET, N. BONNEFOY-BERARD, J. SALGADO, A. AOUACHERIA and J. KUCHARCZAK
Homologous alpha helices from anti-apoptotic Bfl-1 and pro-apoptotic Bax induce mitochondrial permeabilization by non redundant mechanisms
PloS ONE (2012) 7(6):e38620. Epub 2012 Jun 20
- C. HESLING, J. LOPEZ, L. FATTET, P. GONZALO, I. TREILLEUX, D. BLANCHARD, R. LOSSON, V. GOFFIN, N. PIGAT, A. PUISIEUX, I. MIKAELIAN, G. GILLET, and R. RIMOKH
Tif1γ is essential for the terminal differentiation of mammary alveolar epithelial cells and lactation through Smad4 inhibition
Development (2013) 140:167-65
- A. BALLESTA, J. LOPEZ, N. POPGEORGIEV, M. DOUMIC-JAUFFREYand G. GILLET
Data-driven Modeling of Src Control on the Mitochondrial Pathway of Apoptosis.
Implication for Anticancer Therapy Optimization
PloS Comp. Biol. (2013) 9(4): e1003011. doi:10.1371/journal.pcbi.1003011
- FATTET L, AY AS, JALLADES L, GONZALO P, LOPEZ J, MIKAELIAN I, GILLET G, HESLING C, and RIMOKH R.
TIF1γ SUMOylation enhances its repressive activity on TGFβ signaling
J. Cell Science (2013) 126, 3713-23
- I. MIKAELIAN, M. MALEK, J. VIALLET, A. GARCIA, A. GIRARD, C. HESLING, G. GILLET, P. GONZALO, R. RIMOKH and M. BILLAUD
A kinome-wide RNAi screening identifies a novel role for mTORC1 in the maintenance of the epithelial phenotype.
Cancer Res. (2013) 73: 1–11
- J. BIARC, P. GONZALO, I. MIKAELIAN, G. GILLET, R. RIMOKH and J. LEMOINE
Combination of a discovery LC-MS/MS analysis and a label-free quantification for the characterization of an epithelial-mesenchymal transition signature
J. Proteomics (2014) 110 : 183-94
- T. GOULVENT, I. RAY-COQUARD, S. BOREL, V. HADDAD, M. DEVOUASSOUX-SHISHEBORAN, MC. VACHER-LAVENU, E. PUJADE-LAURRAINE, A. SAVINA, D. MAILLET, G. GILLET, I. TREILLEUX and R. RIMOKH
DICER1 and FOXL2 mutations in ovarian sex cord-stromal tumors: a GINECO Group Study.
Histopathology (2015) Jun 1. doi: 10.1111/his.12747.
- L. KASSEM, M. DEYGAS, L. FATTET, J. LOPEZ, T. GOULVENT, E. LAVERGNE, CHABAUD S, N. CARRABIN, N. CHOPIN, T. BACHELOT, G. GILLET, I. TREILLEUX and R. RIMOKH
TIF1γ interferes with TGFβ1/SMAD4 signaling to promote poor outcome in operable breast cancer patients.
BMC Cancer (2015) Jun 4;15:453. doi: 10.1186/s12885-015-1471-y