PUBLICATION
Medial floor plate formation in zebrafish consists of two phases and requires trunk-derived Midkine-a
- Authors
- Schäfer, M., Rembold, M., Wittbrodt, J., Schartl, M., and Winkler, C.
- ID
- ZDB-PUB-050419-11
- Date
- 2005
- Source
- Genes & Development 19(8): 897-902 (Journal)
- Registered Authors
- Rembold, Martina, Schafer, Matthias, Schartl, Manfred, Winkler, Christoph, Wittbrodt, Jochen
- Keywords
- neural patterning; medial floor plate; midkine; pleiotrophin; heparin-binding growth factors; zebrafish
- MeSH Terms
-
- Animals
- Cell Differentiation/physiology*
- Cytokines/metabolism*
- DNA Primers
- Epistasis, Genetic*
- In Situ Hybridization
- Mesoderm/metabolism*
- Models, Biological
- Nerve Growth Factors/metabolism*
- Reverse Transcriptase Polymerase Chain Reaction
- Spinal Cord/embryology*
- Zebrafish/embryology*
- PubMed
- 15833916 Full text @ Genes & Dev.
Citation
Schäfer, M., Rembold, M., Wittbrodt, J., Schartl, M., and Winkler, C. (2005) Medial floor plate formation in zebrafish consists of two phases and requires trunk-derived Midkine-a. Genes & Development. 19(8):897-902.
Abstract
The medial floor plate (MFP) organizes the specification of neurons and outgrowth of axons in the ventral spinal cord of vertebrates. We show that the growth factor Midkine-a, expressed in the paraxial mesoderm, is required for formation of the MFP in zebrafish. Our epistatic analyses demonstrate that development of MFP comprises two independent sequential phases. Following initial MFP induction in the gastrula organizer, Midkine-a regulates allocation of MFP cells during subsequent development. Thus in zebrafish, trunk-derived signals are required for complete MFP formation from a common pool of organizer-derived midline precursor cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping