PUBLICATION
Cloning and characterization of a novel nuclear Bcl-2 family protein, zfMcl-1a, in zebrafish embryo
- Authors
- Chen, M.C., Gong, H.Y., Cheng, C.Y., Wang, J.P., Hong, J.R., and Wu, J.L.
- ID
- ZDB-PUB-001221-5
- Date
- 2000
- Source
- Biochemical and Biophysical Research Communications 279(2): 725-731 (Journal)
- Registered Authors
- Cheng, Ching-Yi, Chen, Ming-chyuan, Gong, Hong-Yi, Hong, Jiann-Ruey, Wang, Jia-Pey, Wu, Jen-Leih
- Keywords
- apoptosis; Bcl-2 family proteins; nuclear Mcl-1; zebrafish; embryogenesis
- MeSH Terms
-
- 3T3 Cells
- Amino Acid Sequence
- Animals
- Base Sequence
- Cloning, Molecular
- Embryo, Nonmammalian/physiology*
- Genes, Reporter
- Green Fluorescent Proteins
- Luminescent Proteins/genetics
- Mice
- Molecular Sequence Data
- Myeloid Cell Leukemia Sequence 1 Protein
- Neoplasm Proteins/chemistry
- Neoplasm Proteins/genetics*
- Neoplasm Proteins/isolation & purification
- Protein Structure, Secondary
- Proto-Oncogene Proteins c-bcl-2/chemistry
- Proto-Oncogene Proteins c-bcl-2/genetics*
- Proto-Oncogene Proteins c-bcl-2/isolation & purification
- Rats
- Recombinant Proteins/chemistry
- Sequence Alignment
- Sequence Homology, Amino Acid
- Transfection
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish Proteins
- PubMed
- 11118352 Full text @ Biochem. Biophys. Res. Commun.
Citation
Chen, M.C., Gong, H.Y., Cheng, C.Y., Wang, J.P., Hong, J.R., and Wu, J.L. (2000) Cloning and characterization of a novel nuclear Bcl-2 family protein, zfMcl-1a, in zebrafish embryo. Biochemical and Biophysical Research Communications. 279(2):725-731.
Abstract
The importance of the Bcl-2 family proteins in normal vertebrate embryogenesis is being recognized; however, their regulatory mechanism is poorly understood. To elucidate the embryonic roles of Bcl-2 family proteins, we cloned and characterized the first zebrafish Bcl-2 family protein, zfMcl-1a. Zebrafish Mcl-1a shows the highest homology to rat Mcl-1 and contains several conserved BH domains of the Bcl-2 family proteins. It also contains a nuclear localization signal (NLS). Using EGFP reporter analysis, we verified the nuclear localization of zfMcl-1a. Deletion of the NLS resulted in distribution of the fusion protein in the cytoplasm. Northern blot analysis indicated that zfMcl-1a mRNA is 1.5 kb and was expressed in oocytes and throughout embryonic development. Notably, the expression of zfMcl-1a transcript was significantly downregulated during gastrulation. These results suggest that zfMcl-1a is a novel nuclear Bcl-2 family protein and is likely to play an important role in zebrafish oogenesis and embryogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping