PUBLICATION

The teleostean (zebrafish) dopaminergic system ascending to the subpallium (striatum) is located in the basal diencephalon (posterior tuberculum)

Authors
Rink, E. and Wullimann, M.F.
ID
ZDB-PUB-001220-2
Date
2001
Source
Brain research   889: 316-330 (Journal)
Registered Authors
Rink, Elke, Wullimann, Mario F.
Keywords
tyrosine hydroxylase; dopamine; nigrostriatal system; posterior tuberculum; teleosts; zebrafish
MeSH Terms
  • Animals
  • Coloring Agents
  • Diencephalon/anatomy & histology*
  • Diencephalon/enzymology
  • Diencephalon/physiology*
  • Dopamine/physiology*
  • Fluorescent Dyes
  • Immunohistochemistry
  • Lysine/analogs & derivatives
  • Neostriatum/anatomy & histology*
  • Neostriatum/enzymology
  • Neostriatum/physiology*
  • Tyrosine 3-Monooxygenase/metabolism
  • Zebrafish/physiology*
PubMed
11166725 Full text @ Brain Res.
Abstract
Tyrosine hydroxylase immunohistochemistry is used to demonstrate catecholaminergic neuronal populations in the fore- and midbrain of adult zebrafish (Danio rerio). While no catecholaminergic neurons are found in the midbrain, various immunoreactive populations were found in the diencephalon (hypothalamus, posterior tuberculum, ventral thalamus, pretectum) and telencephalon (preoptic region, subpallium, olfactory bulb). The posterior tubercular catecholaminergic cells include three cytological types (small round, large pear-shaped, and bipolar liquor-contacting cells). Furthermore, the retrograde neuronal tracers DiI or biocytin were applied to demonstrate ascending projections to the basal telencephalon (incl. the striatum). A double-label approach was used - together with tyrosine hydroxylase immunohistochemistry - in order to visualize neurons positive for tyrosine hydroxylase and a retrograde tracer. Double-labeled cells were identified in two locations in the posterior tuberculum (i.e, small round neurons in the periventricular nucleus of the posterior tuberculum and large pear-shaped cells adjacent to it). They are interpreted as the teleostean dopaminergic system ascending to the striatum, since previous work [16] established that no noradrenergic neurons exist in the forebrain of the adult zebrafish.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping