Morpholino

MO1-mir218

ID
ZDB-MRPHLNO-110628-4
Name
MO1-mir218
Previous Names
  • miR-218 MO1 (1)
Targets
Sequence
5' - TGCATGGTTAGATCAAGCACAAGGG - 3'
ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
This morpholino is designed with "wobble" so that it can target all three copies of mir218 (mir218a-1, mir218a-2, and mir218b).
Genome Resources
None
Target Location
Genomic Features
No data available
Expression
Gene expression in Wild Types + MO1-mir218
Expressed Gene Anatomy Figures
mir218a-1 Fig. 2 with image from Fish et al., 2011
robo1 Fig. 3 with image from Fish et al., 2011
slit2 Fig. 2 with image from Fish et al., 2011
slit3 Fig. 2 with image from Fish et al., 2011
Phenotype
Phenotype resulting from MO1-mir218
No data available
Phenotype of all Fish created by or utilizing MO1-mir218
Phenotype Fish Conditions Figures
blood circulation decreased process quality, abnormal s843Tg; sd2Tg + MO1-mir218 standard conditions Fig. 2 with image from Fish et al., 2011
atrium malformed, abnormal s896Tg; twu26Tg + MO1-mir218 standard conditions Fig. 2 with image from Fish et al., 2011
cardiac muscle tissue morphogenesis decreased process quality, abnormal s896Tg; twu26Tg + MO1-mir218 standard conditions Fig. 3 with image from Fish et al., 2011
endocardium mislocalised, abnormal s896Tg; twu26Tg + MO1-mir218 standard conditions Fig. 3 with image,  Fig. 4 with image from Fish et al., 2011
endocardium morphogenesis decreased process quality, abnormal s896Tg; twu26Tg + MO1-mir218 standard conditions Fig. 3 with image from Fish et al., 2011
cardioblast migration to the midline involved in heart field formation decreased process quality, abnormal s896Tg; twu26Tg + MO1-mir218 standard conditions Fig. 3 with image from Fish et al., 2011
myocardium mislocalised, abnormal s896Tg; twu26Tg + MO1-mir218 standard conditions Fig. 3 with image from Fish et al., 2011
cardiac ventricle malformed, abnormal s896Tg; twu26Tg + MO1-mir218 standard conditions Fig. 2 with image from Fish et al., 2011
cardioblast migration decreased process quality, abnormal s896Tg; twu26Tg + MO1-mir218 standard conditions Fig. 4 with image from Fish et al., 2011
cardioblast migration delayed, abnormal s896Tg; twu26Tg + MO1-mir218 + MO1-robo1 standard conditions Fig. 4 with image from Fish et al., 2011
Citations
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