Morpholino

MO3-tbxta

ID

ZDB-MRPHLNO-071130-1

Name

MO3-tbxta

Previous Names

MO3-ntl
MO3-ta

Target

tbxta (1)

Sequence

5' - GACTTGAGGCAGACATATTTCCGAT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

Published as a photoactivatable morpholino. A photo-cleavable version of this MO was described in Tallafuss et al. 2012 (ZDB-PUB-120412-12). MO nucleotide 13 (A) was replaced by a photo-sensitive subunit. A caged version of this morpholino was described in Darrah et al., 2021( ZDB-PUB-211028-5). A self-transfecting guanidinium linked morpholino (GMO) was described in Das et al., 2023 ZDB-PUB-230420-69.

Genome Resources

None

Target Location

Genomic Features

No data available

Expression

Gene expression in Wild Types + MO3-tbxta

Expressed Gene	Anatomy	Figures
myh9a	primitive heart tube	Fig. 4 from Veerkamp et al., 2013

Phenotype

Phenotype resulting from MO3-tbxta

Phenotype	Fish	Figures
determination of heart left/right asymmetry decreased process quality, abnormal	twu34Tg + MO3-tbxta	Fig. 4 from Veerkamp et al., 2013
primitive heart tube bilateral symmetry, abnormal	twu34Tg + MO3-tbxta	Fig. 4 from Veerkamp et al., 2013

Phenotype of all Fish created by or utilizing MO3-tbxta

Phenotype	Fish	Conditions	Figures
notochord development disrupted, abnormal	WT + MO3-tbxta	ultraviolet light	Fig. S2 from Shestopalov et al., 2007
notochord aplastic, abnormal	WT + MO3-tbxta	ultraviolet light	Fig. S2 from Shestopalov et al., 2007
notochord morphology, abnormal	WT + MO3-tbxta	ultraviolet light	Fig. 2, Fig. S2 from Shestopalov et al., 2007
somite U-shaped, abnormal	WT + MO3-tbxta	ultraviolet light	Fig. 2, Fig. S2 from Shestopalov et al., 2007
post-vent region malformed, abnormal	WT + MO3-tbxta	ultraviolet light	Fig. 1, Fig. S2 from Shestopalov et al., 2007
primitive heart tube bilateral symmetry, abnormal	twu34Tg + MO3-tbxta	standard conditions	Fig. 4 from Veerkamp et al., 2013
determination of heart left/right asymmetry decreased process quality, abnormal	twu34Tg + MO3-tbxta	standard conditions	Fig. 4 from Veerkamp et al., 2013

Citations