Morpholino

MO1-six3a,six3b

ID

ZDB-MRPHLNO-051220-2

Name

MO1-six3a,six3b

Previous Names

Targets

Sequence

5' - GCTCTAAAGGAGACCTGAAAACCAT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The authors state that this morpholino is also effective at reducing translation of six3a, to which it has a 2-bp mismatch.

Genome Resources

Target Location

Genomic Features

No data available

Expression

Gene expression in Wild Types + MO1-six3a,six3b

Expressed Gene	Anatomy	Figures
eomesa	telencephalon	Fig. 5 from Ando et al., 2005
lhx2b	forebrain neural keel	Fig. 4 from Ando et al., 2005
myod1	segmental plate somite 1	Fig. 4 from Ando et al., 2005
pax2a	midbrain hindbrain boundary midbrain hindbrain boundary neural keel optic primordium optic stalk	Fig. 4 , Fig. 5 from Ando et al., 2005

Phenotype

Phenotype resulting from MO1-six3a,six3b

No data available

Phenotype of all Fish created by or utilizing MO1-six3a,six3b

Phenotype	Fish	Conditions	Figures
retina sensory epithelium regeneration decreased occurrence, abnormal	WT + MO1-six3a,six3b	light ablation: photoreceptor cell	Fig. 5 from Lenkowski et al., 2013
retina stem cell proliferation decreased occurrence, abnormal	WT + MO1-six3a,six3b	light ablation: photoreceptor cell	Fig. 5 from Lenkowski et al., 2013
hypothalamus anterior region mCherry expression absent, abnormal	zf994Tg; zf997Tg + MO1-six3a,six3b (WIK/AB)	control	Fig. 2 from Bhatia et al., 2015
hypothalamus posterior region mCherry expression decreased distribution, abnormal	zf994Tg; zf997Tg + MO1-six3a,six3b (WIK/AB)	control	Fig. 2 from Bhatia et al., 2015
hypothalamus anterior region EGFP expression absent, abnormal	zf994Tg; zf997Tg + MO1-six3a,six3b (WIK/AB)	control	Fig. 2 from Bhatia et al., 2015
hypothalamus posterior region EGFP expression decreased distribution, abnormal	zf994Tg; zf997Tg + MO1-six3a,six3b (WIK/AB)	control	Fig. 2 from Bhatia et al., 2015
hindbrain mCherry expression amount, ameliorated	zf3589Tg; zf3590Tg + MO1-six3a,six3b	standard conditions	Fig 2 from Bengani et al., 2021
midbrain mCherry expression amount, ameliorated	zf3589Tg; zf3590Tg + MO1-six3a,six3b	standard conditions	Fig 2 from Bengani et al., 2021

Citations