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Fig. 1 Multiorgan response to cardiac injury in mouse and zebrafish by scRNA-Seq.Experimental outline for mouse (a) or zebrafish (b) heart injury and sampling for scRNA-seq analysis. A scRNA-seq analysis was done per sample, for a total of 28 murine samples (5 tissues, 3 timepoints except for the kidney which were analyzed at 7 and 30 dpi, and 2 conditions, sham and MI. Each sample included cells from one or two biological replicates combined 1:1). 24 zebrafish samples (4 tissues, 3 conditions, and 2 biological replicates) were included. a was created with BioRender. c UMAPs (Uniform manifold approximation and projection) of the 194,315 cells (from 41 mice; 22 sham and 19 MI) colored by timepoint (left), tissue of origin (center) and cell type (right). Dpi, days post infarction. d UMAP of 70,783 zebrafish cells showing treatments timepoints (left), tissue of origin (center) and cell type (right). Dpci, days post cardiac injury; WKM, whole kidney marrow; Ctrl, uninjured. Bar plot of DEGs (MI compared to sham procedure) in mice (e) and zebrafish (f) by tissue and cell type. Bars are colored by timepoint. Genes with FDR < 0.05 and absolute log2(FC) > 0.25 were considered differentially expressed.