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Figures for Abbas et al., 2024
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Figure 1

Western blot analysis of the temporal expression pattern of the short Gαs isoform (GαsS) in whole wild-type zebrafish from 24 to 96 h post-fertilization (hpf). (a) Representative blot of the GαsS expression level using GAPDH as a loading control. Whole zebrafish tissue lysates were used to extract total protein (~40 µg loaded per well). (b) The band intensities were quantified using ImageJ software, followed by normalization of the GαsS band intensity to that of GAPDH, yielding the relative GαsS expression level in arbitrary units. One-way ANOVA followed by Tukey’s multiple comparisons test were performed (* p < 0.05). Each bar represents the mean ± SEM (n = 3).

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