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Figure Caption
Fig. 3 Testosterone concentration and uptake in zebrafish embryos. Wild-type embryos at 3 h post-fertilization (hpf) were exposed to vehicle (0.1% DMSO) or testosterone (30 ?M) until 3 days post-fertilization (dpf). (A,B) Testosterone levels were measured using ELISA to calculate nM testosterone per embryo (A) and percentage of testosterone uptake per embryo (B). Each circle represents the mean testosterone concentrations or uptake per embryo for a single clutch of embryos, with 50 embryos pooled per clutch. Horizontal lines are the mean of each clutch.
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