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Figure 2 Rescue of ovl mutants with Tg(hsp70l:ift88-GFP) transgene. (A) Schematic diagram of hsp70l:ift88-GFP construct. (B) Procedure of heat shock experiments for ovl mutants rescue assay. hphs, hour post heat shock. (C) External pheontype of 5 dpf wild type, ovl mutant or ovl mutant larvae carrying Tg(hsp70l:ift88-GFP) transgene. The numbers of larvae investigated were shown on the bottom right. (D) Confocal images showing cilia in different type of organs as indicated. Red channel indicates cilia visualized by anti-acetylated ?-tubulin antibody and fluorescence of Tg(hsp70l:ift88-GFP) is showed in green. Scale bars: 200 ?m in panel C and 10 ?m in panel D.