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Fig. 1 Samd7 is expressed in immature photoreceptors, red cones, green cones, and rods in the larval zebrafish retina. (A) UMAP (Uniform Manifold Approximation and Projection) of scRNA-seq data from 4-dpf crx:GFP+ photoreceptors. (B) scRNA-seq results show that samd7 is restricted to immature photoreceptors, red cones, green cones, and rods. See SI Appendix, Fig. S1 for detailed expression patterns. (C?K) Analysis of samd7 expression in the 3-dpf larval eye by fluorescent in situ hybridization. (C) Optical cross-section of the peripheral larval retina stained with probes against samd7 (green) and red/green-cone-specific arr3a (red) shows that samd7 is expressed in immature arr3a? photoreceptors (?immature photoreceptors?). (D) Whole-mount view of the eye in C. (E) Close-up view of the dotted square in D shows samd7+ puncta in arr3a+ red and green cone cell bodies. (F) Optical cross-section of the peripheral larval retina stained with probes against samd7 (green) and blue/UV-cone-specific arr3b (red). (G) Whole-mount view of the eye in F. (H) Close-up view of the dotted square in G shows that samd7 is not expressed in arr3b+ blue and UV cone cell bodies. (I) Optical cross-section of the peripheral larval retina stained with probes against samd7 (green) and rod-specific gnat1 (red). (J) Whole-mount view of the same eye as in I. (K) Close-up view of the dotted square in J shows samd7+ puncta in gnat1+ rod cell bodies. n = 3 eyes analyzed per in situ. [Scale bar in (C, D, F, G, I, and J), 50 µm; Scale bar in (E, H, and K), 5 µm.] D, V, N, T: Dorsal, Ventral, Nasal, Temporal. ONH; optic nerve head. ONL; outer nuclear layer.