Figure 2.

Figure 2.

Locomotion defects observed in spastizin mutants during unmotivated and counter-current trials. Data are represented as box plots for assessing various aspects of locomotive ability of wild-type and spastizin mutants. The orange line shows the median of all individuals, the box displays the upper and lower quartile, the whiskers denote 1.5 times the interquartile range, the plus-sign marks outlier. (a) The homozygous mutants spend less time in the centre of the water current as compared to heterozygous and wild-type fish. (b) Each histogram is normalized to 100% and the colour bar goes from 0 to1%. The heat map shows all possible locations of the fish in the setup. Blue colour indicates low probability and red colour illustrates high probability of presence. Whereas the spastizin mutants are more dispersed within the setup, the wild-type fish stay more at the centre, where the current is most constant. (c) Decrease in the median thrust velocity of homozygous spastizin mutants as compared to heterozygous and wild-type fish during unmotivated trials. (d) Increase in the bending angle for both heterozygous and homozygous mutants compared to wild-types. (e) Increase in pectoral bending peak position (normalized to body length) of heterozygous and homozygous mutants compared to wild-types. (f) Computational model depicting a shift in the peak position of pectoral standing wave, which compensates for the dampened travelling wave. The box plot shows the model performance with fully flexible and compensated at the same level and high as compared to the stiff (no. of fish: wt = 53, +/− = 23, −/− = 21). Statistical significance was tested with Fisher’s permutation test. *p < 0 .05, **p < 0.01, ***p < 0.001.