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Fig. 7 vapb knock-out does not phenocopy VapBP56S or WT VapB transgenic expression. A, vapb exon 1 with deletion in the vapb mutant in red. Guide RNAs used to generate line are shown. This mutation eliminates the start site for protein translation (shown in yellow). B, Anti-VapB Western blot of whole larval protein extracts from WT, heterozygous siblings, and vapb mutants. C?E, Images of the pLL ganglion (outlined) of HCR RNA FISH labeling vapb mRNA in WT (C), heterozygous (D; vapb+/?), or vapb mutant (E; vapb?/?) animals. F, Quantification of vapb mRNA mean fluorescence intensity normalized to WT. G?I, Images of the pLL ganglion (outlined) of HCR RNA FISH labeling the vapb homolog vapal. WT (G), heterozygous (H; vapb+/?), and vapb mutant (I; vapb?/?) animals are shown. J, Quantification of vapal mRNA mean fluorescence intensity normalized to WT. K?M, Images of HCR RNA FISH labeling tfam mRNA in the pLL ganglion (outlined) in WT (K), heterozygous (L; vapb+/?), or vapb mutant (M; vapb?/?) animals. N, Quantification of tfam mean fluorescence intensity normalized to WT. O, P, Representative images of axon terminals expressing the mitophagy indicator in WT (O) and vapb?/? animals (P). Distribution of acidified (magenta) and unacidified (outline) mitochondria illustrated (O```?P```). Q, Proportion of acidified mitochondrial area relative to total mitochondrial population area. Kruskal?Wallis with Dunn's post hoc contrasts for F, J. ANOVA with Dunnett's post hoc contrasts in N, Q. Scale bar, 10 ?m in C, G, and K, 5 ?m in O``. Each data point for F, J, N, and Q represents the average calculated from an individual animal. All data represented as mean ± SEM.