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Fig. 5 Ints1-deficient zebrafish larvae exhibit increased dbh mRNA expression in the locus coeruleus but not in the medulla oblongata. (A) Representative image, showing of dbh mRNA expression by whole-mount in situ hybridization of 5 dpf Ints1-deficient (right) and WT sibling (left) larvae (dorsal views). The locus coeruleus is encircled (green). (B) Quantification of the dbh mRNA signal in the locus coeruleus reveals significantly elevated expression in Ints1-deficient (−/−) larvae compared with their WT siblings (**P<0.01, two-tailed t-test assuming equal variance; n=7). (C) Quantification of dbh mRNA levels in the medulla oblongata shows no difference between Ints1-deficient larvae and their WT siblings (n=7). Error bars in B and C represent ±s.e.