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Fig. 3 Loss of myo7aa causes hearing impairment. (A,B) YO-PRO-1 fluorescence staining results of wild-type and myo7aa mutant hair cells at 5 dpf, arrowheads indicate zebrafish inner ear (red) and lateral line hair cells (blue); (C,D) YO-PRO-1 fluorescent staining results of ear hair cells in wild-type and myo7aa mutant at 5 dpf, arrowheads indicate zebrafish inner ear hair cells (red) and otoliths (yellow). These results demonstrate the impact of myo7aa loss on the function of hair cells in the ear, leading to hearing impairment. Additionally, the results of genotype identification of embryos shown in (B,D) are presented. (E) Genotyping results of line2. M is DNA marker, WT indicates wild type, 1–8 are embryos shown in (B,D). (F,G) The movement distance (F) and reaction speed (G) of wild type and myo7aa mutant within 0.3 s after acoustic stimulation at 5 dpf are measured; n = 20; mean with SEM; with t-test, **** indicates a p-value <0.0001.