ZFIN Image: Hammond et al., 2024, Figure 7.

Image

Figure Caption

Figure 7. trib1 and trib3 overexpression do not induce expression of the Hif reporter phd3:GFP.

(A) Stereo-fluorescence micrographs of 2 dpf phd3:GFP larvae injected with phenol red (PR), dominant active hif-1α (DA1) and dominant negative Hif-1α (DN1) RNA controls alongside trib1 (T1) and trib3 (T3) RNA. Scale bars = 200 µM. (B) Corrected fluorescence intensity levels of phd3:GFP (Hif reporter) larvae shown in (A). Data shown are mean ± standard error of the mean (SEM), n = 30 embryos accumulated from three independent experiments. Statistical significance determined through one-way analysis of variance (ANOVA) with multiple comparisons. p values shown are: ***p < 0.001.

Acknowledgments

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