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Fig. 5 Removal of maternal pomt1 mRNA contribution from oocytes leads to earlier phenotypes in pomt1 KOKO larvae. (A) Residual Pomt1 is present in 5 dpf KOHet larvae derived from Het X Het crosses, as is α-DG glycosylation. (B) Both Pomt1 and α-DG glycosylation are absent at 5 dpf in KOKO larvae obtained from KO mothers crossed with Het fathers. (C) Survival in pomt1 KOKO zebrafish is reduced starting at 10 dpf as shown by the deviation from expected Mendelian ratios (50% for HetKOs and KOHets) in 3 independent clutches. (D). Reduced body size is observed in KOKO larvae and juveniles starting at 10 dpf. ***p < 0.001, *p < 0.05. Alt-text. This figure shows that pomt1 knock-out larvae from heterozygous mothers at 5 dpf still have Pomt1 present and that alpha-dystroglycan is glycosylated. When knock-out larvae are obtained from knock-out mothers both Pomt1 and alpha-dystroglycan glycosylation are absent. pomt1 knock-out larvae from knock-out mothers begin to die at 10 dpf and are significantly smaller than their clutch mates at the same age