Figure 2

Figure 2

Intrahepatic liver vasculature was altered in Dhx15^+/− mice. (A) Representative Cd31 liver immunostaining (green) for wild-type (panel a) and Dhx15^+/− (panel b) mouse. Nuclei counterstaining was performed with DAPI (blue). Confocal microscope, original magnification: 300×. (B) RNA extraction of liver tissue from either wild-type or Dhx15^+/− mice was performed. mRNA expression was analyzed by RT-qPCR. The graphs show the expression levels of Vegf-a, Vegf-c, Vegf-d, Vegfr1, Vegfr3, Podoplanin, and Angiopoietin 1 genes in the wild-type and Dhx15^+/− conditions. mRNA levels are shown as fold change relative to Hprt mRNA levels. Bars represent mean ± SEM, * p < 0.05 vs. wild-type (n = 4). (C) RNA extraction of the hepatocyte cell line without or with silenced Dhx15 gene was performed. mRNA expression was analyzed by RT-qPCR. The graphs show the expression levels of Vegf-a, Vegf-c, Vegf-d, and Angiopoietin 1 genes in wild-type and Dhx15 silenced conditions. mRNA levels are shown as fold change relative to Hprt mRNA levels. Bars represent mean ± SEM, * p < 0.05 vs. wild-type (n = 4).