Fig. 7

Fig. 7 METTL3 deficiency inhibits Wnt/β-catenin signaling and Wnt/β-catenin activation partially alleviates the phenotypes of mettl3 morphants.

A–C Dual luciferase assay demonstrating the effect of SuperTop/SuperFop reporter activity in BMSCs, HEPM cells, and DPSCs transfected with the shMETTL3 vector. D–F Western blot showing the protein levels of TCF1, GSK3, phosphorylated-GSK3, β-catenin, and phosphorylated-β-catenin in control and stable METTL3 knockdown cells, GAPDH was used as a loading control. G, H Quantitative analyses of the relative expression of phosphorylated GSK3 and β-catenin. I Schematic of the experimental design to assess the effect of CHIR99021 at different doses. J Wnt/β-catenin activation partially alleviates the phenotypes of mettl3 morphants. Results were presented as mean ± SD of three independent experiments. *P < 0.05, **P < 0.01 or ***P < 0.001 indicates a significant difference between the groups.