Fig. 6

Fig. 6 PSEN1 is specifically recognized by YTHDF1 and directly interacts with β-catenin.

A Schematic of the design for the RNA pull-down assay. B Immunoblotting of YTHDF1 after the RNA pull-down assay with cell lysate, biotinylated-PSEN1, and biotinylated-control in the cells. C Schematic of the design for the RNA immunoprecipitation (RIP) assay. D RIP assay to determine the enrichment of PSEN1 in cells incubated with anti-YTHDF1 antibody. E–H Cells were transiently transfected with control, siYTHDF1, empty vector, or OEYTHDF1, respectively. The half-life (t_1/2) of the PSEN1 mRNA was measured. I Silver staining revealed PSEN1-bound proteins in BMSCs, HEPM cells and DPSCs. J Interaction between β-catenin and PSEN1 determined by co-IP followed by western blot analysis. K Representative image showing the enrichment of PSEN1 and β-catenin after METTL3 knockdown by immunostaining analyses in BMSCs, HEPM cells, and DPSCs. L Schematic diagram showing the mechanism of PSEN1. Results were presented as mean ± SD of three independent experiments. ***P < 0.001 indicates a significant difference between the groups.