Fig. 3

Fig. 3 Identification of METTL3 targets via MeRIP-seq and RNA-seq.

A Schematic diagram depicting the protocols used for MeRIP-seq and RNA-seq. B Volcano plot of differentially expressed genes between METTL3 knockdown and control BMSCs. C Flow chart showing the shared downregulated genes with hypomethylated m⁶A peaks. D Gene enrichment analysis performed via the Metascape database. E KEGG pathway enrichment analysis shows major signaling pathways in METTL3-knockdown BMSCs compared to control BMSCs. F The expression level of PSEN1 in BMSCs, HEPM cells, and DPSCs in the METTL3-knockdown and control groups. G The m⁶A abundances of psen1 transcript in mettl3-knockdown zebrafish embryos compared to control embryos. H Validation of m⁶A modification in METTL3-knockdown and control cells using MeRIP-qPCR. Results were presented as mean ± SD of three independent experiments. *P < 0.05, **P < 0.01 or ***P < 0.001 indicates a significant difference between the groups.