Figure 4

Figure 4 nomo1^−/− zebrafish displayed social deficiency and repetitive behaviors.

(A–E) The social preference experiment with 2-mpf zebrafish. (A) Schematic diagram of the individual social behavior experiment. A heatmap (B, C) showing that nomo1^−/− zebrafish spent significantly less time in the social area than WT zebrafish. The ratio of distance from the social area (D) and time spent in the social area (E) was significantly lower for nomo1^−/− zebrafish than for WT zebrafish (biological replicates, N = 20). (F, G) Schematic of the shoaling test in which the interindividual distance exhibited by nomo1^−/− zebrafish was significantly higher than WT zebrafish (biological replicates, N = 4). (H, I) Schematic of different repetitive behaviors including “back-and-forth motions”, “small circling” and “big circling”. nomo1^−/− zebrafish exhibited a significantly higher proportion of all kinds of repetitive behaviors (biological replicates, N = 16). Data information: Data are analyzed using unpaired t test and presented as the mean ± SEM, ^*P < 0.05. Source data are available online for this figure.