ZFIN Image: Yang et al., 2023, Fig. 2

Image

Figure Caption

Fig. 2 Malformation of pharyngeal cartilages in nkx2.3 mutants. (A) Knockout of nkx2.3 by CRISPR-Cas9 system. The nkx2.3△76 allele was identified by the targeting of the second exon of the nkx2.3 locus (top panel), which carried a 76-bp deletion resulting in a premature stop codon. The truncated protein in nkx2.3△76 mutants only has 130 amino acids (bottom panel). (B) Morphology of nkx2.3 mutant embryos at indicated stages. Scale bars, 200 μm. (C) Photomicrographs of the cartilages stained with Alcian blue. The cartilages were shown anterior to the left and included the ceratobranchial (cb), ceratohyal (ch), Meckel's cartilage (m), and palatoquadrate (pq) cartilages. Scale bars, 100 μm.

Figure Data

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Heliyon