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Fig. 3 Expression of cdt1 in zebrafish. (a) Quantitative real-time polymerase chain reaction (qPCR) analysis of expression levels of the cell division cycle 10 (Cdc10)-dependent transcript 1 (cdt1)messenger RNA (mRNA) in maternal, 4-cell stage to 5 dpf zebrafish embryos. (b) Representative whole-mount in situ hybridization (WISH) images showing expression patterns of cdt1 mRNA in maternal and 1 to 5 dpf zebrafish embryos. (c) Western blot analysis of expression levels of Cdt1 protein in maternal and 1 to 5 dpf zebrafish embryos. Top panel: Cdt1 antibody; middle panel: β-tubulin antibody; bottom panel: Coomassie blue staining. Negative control: cdt1 mutant (mu); positive control: Myc-Cdt1 overexpression in 293T cells; CK: empty vector; dpf: days post fertilization; WT: wild type.